Photodynamic inactivation for in vitro decontamination of Staphylococcus aureus in whole blood

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorCorrea, Thaila Quatrini-
Autor(es): dc.creatorBlanco, Kate Cristina-
Autor(es): dc.creatorSoares, Jennifer Machado-
Autor(es): dc.creatorInada, Natalia Mayumi-
Autor(es): dc.creatorKurachi, Cristina-
Autor(es): dc.creatorGolim, Marjorie de Assis [UNESP]-
Autor(es): dc.creatorDeffune, Elenice [UNESP]-
Autor(es): dc.creatorBagnato, Vanderlei Salvador-
Data de aceite: dc.date.accessioned2022-02-22T00:15:30Z-
Data de disponibilização: dc.date.available2022-02-22T00:15:30Z-
Data de envio: dc.date.issued2020-12-10-
Data de envio: dc.date.issued2020-12-10-
Data de envio: dc.date.issued2019-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2019.08.013-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197600-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197600-
Descrição: dc.descriptionBackground: Blood can be the target of microbial cells in the human body. Erythrocytes, platelets, and plasma concentrates in blood bags used in hemotherapy for blood transfusion are contamination targets, which can trigger serious diseases in blood. These infections can cause septicemia that can lead to death if not recognized rapidly and treated adequately. The aim of this study was to evaluate the photodynamic inactivation in the in vitro decontamination of Staphylococcus aureus in whole blood, erythrocytes and platelet-rich plasma. Methods: Photodynamic inactivation using light doses of 10, 15 and 30 J/cm(2) at 630 nm and an hematoporphyrin-derivative photosensitizer (Photogem (R)) solutions at 25 and 50 mu g/mL were evaluated. Toxicity of treatment was determined by hemolysis and cell viability assays. Results: The S. aureus reduction in phosphate buffered saline (PBS), whole blood, erythrocytes and platelet-rich plasma at 15 J/cm(2) and 50 mu g/mL were 7.2, 1.0, 1.3 and 0.4 log CFU/mL, respectively. Quantitative and qualitative analyses were performed in whole blood samples, and Photogem (R) showed a low risk of hemolysis (10.7%) in whole blood. However, 100% of erythrocytes suffered hemolysis in the absence of plasma. The cell viability assay showed 13.9% of apoptosis in erythrocytes, but normal platelet viability. Conclusion: S. aureus inactivation of whole blood samples using 50 mu g/mL Photogem (R) and 15 J/cm(2) resulted in better outcomes, providing promising indications for treatment of bacterial contamination of blood, and in this work, alternative possibilities to apply the technique for blood decontamination are discussed.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Fed Sao Carlos, PPG Biotec, BR-13565905 Sao Carlos, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Sao Carlos Inst Phys, POB 369,Av Trabalhador Sao Carlense, BR-13565905 Sao Carlos, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Botucatu Med Sch, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Botucatu Med Sch, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2013/07276-1-
Descrição: dc.descriptionCAPES: 1500213-
Formato: dc.format58-64-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationPhotodiagnosis And Photodynamic Therapy-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectPhotodynamic inactivation-
Palavras-chave: dc.subjectBlood-
Palavras-chave: dc.subjectDecontamination-
Palavras-chave: dc.subjectStaphylococcus aureus-
Título: dc.titlePhotodynamic inactivation for in vitro decontamination of Staphylococcus aureus in whole blood-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.