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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Univ Santo Amaro | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.creator | Borges, Marcelo Sant'Ana | - |
Autor(es): dc.creator | Barbosa Born, Joana Larissa | - |
Autor(es): dc.creator | Conti, Leticia Martins | - |
Autor(es): dc.creator | Segabinazzi, Lorenzo Garrido [UNESP] | - |
Autor(es): dc.creator | Nichi, Marcilio | - |
Autor(es): dc.creator | Vechiato Kawai, Giulia Kiyomi | - |
Autor(es): dc.creator | Leite, Roberta Ferreira | - |
Autor(es): dc.creator | Peixoto, Kleber da Cunha | - |
Autor(es): dc.creator | Dell'Aqua, Jose Antonio [UNESP] | - |
Autor(es): dc.creator | Papa, Frederico Ozanam [UNESP] | - |
Autor(es): dc.creator | Crespilho, Andre Maciel | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:11:57Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:11:57Z | - |
Data de envio: dc.date.issued | 2020-12-09 | - |
Data de envio: dc.date.issued | 2020-12-09 | - |
Data de envio: dc.date.issued | 2020-05-01 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/197341 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/197341 | - |
Descrição: dc.description | BACKGROUND: Some antioxidants have been used in semen extenders to reduce adverse effects caused by excessive reactive oxygen species (ROS) production. The study was carried out to assess the effect of quercetin (QC) antioxidant therapy on goat semen submitted to cryopreservation. OBJECTIVE: To evaluate the effect of quercetin incorporation in different phases of the cryopreservation process of goat spermatozoa. MATERIALS AND METHODS: Five ejaculates from each of four goats (n=20) were collected and split into four groups: Control (G1), without QC; G2, 15 mu M of QC added to semen before centrifugation; G3, 15 mu M QC added to semen after centrifugation; G4, 15 mu M QC added to semen before centrifugation and 15 mu M of QC added to semen after centrifugation (total of 30 mu M of QC); and cryopreserved. All semen samples were evaluated after thawing for sperm kinetics, plasma membrane integrity, and ROS levels. RESULTS: Although lower concentrations of ROS were associated with groups that received antioxidant supplementation (P=0.0213), linear and dose dependent (P<0.05) reductions of the total and progressive sperm motility, velocity and percentage of fast cells were related to the QC groups. Likewise, plasma membrane integrity was better preserved (P=0.0154) in the control group (35.5%) than in groups that received QC (G2=32.6%, G3=32.4% and G4=26.7%). CONCLUSION: Although quercetin was efficient at reducing the oxidative stress related to sperm cryopreservation, it exerted a deleterious dose-dependent effect on the kinetics and integrity of the frozen goat semen, contradicting its use at the tested concentrations. | - |
Descrição: dc.description | Univ Santo Amaro, UNISA, Sao Paulo, SP, Brazil | - |
Descrição: dc.description | Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil | - |
Descrição: dc.description | Univ Sao Paulo, Fac Vet Med & Anim Sci FMVZ, Dept Anim Reprod VRA, Sao Paulo, Brazil | - |
Descrição: dc.description | Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil | - |
Formato: dc.format | 128-134 | - |
Idioma: dc.language | en | - |
Publicador: dc.publisher | Cryo Letters | - |
Relação: dc.relation | Cryoletters | - |
???dc.source???: dc.source | Web of Science | - |
Palavras-chave: dc.subject | antioxidant | - |
Palavras-chave: dc.subject | cryopreservation | - |
Palavras-chave: dc.subject | goat | - |
Palavras-chave: dc.subject | quercetin | - |
Palavras-chave: dc.subject | sperm quality | - |
Título: dc.title | PARADOXICAL EFFECT OF QUERCETIN ANTIOXIDANT ON GOAT SPERM PARAMETERS AFTER CRYOPRESERVATION | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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