Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA

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Autor(es): dc.contributorInst Ciencias Biomed-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Santo Amaro-
Autor(es): dc.contributorInst Adolfo Lutz Registro-
Autor(es): dc.contributorFdn Ezequiel Dias-
Autor(es): dc.creatorLourenco, Alexandre-
Autor(es): dc.creatorDurigon, Edison Luis-
Autor(es): dc.creatorZanotto, Paolo-
Autor(es): dc.creatorGazzinelli Cruz Madeira, Jovita Eugenia-
Autor(es): dc.creatorDe Almeida, Adriana Palma-
Autor(es): dc.creatorCorrea, Benedito-
Data de aceite: dc.date.accessioned2022-02-22T00:11:49Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:49Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2007-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S0074-02762007005000102-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197298-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197298-
Descrição: dc.descriptionAspergillus flavus is a very important toxigenic fungus that produces aflatoxins, a group of extremely toxic substances to man and animals. Toxigenic fungi can grow in feed crops, such as maize, peanuts, and soybeans, being thus of high concern for public health. There are toxigenic and non-toxigenic A. flavus variants, but the necessary conditions for expressing the toxigenic potential are not fully understood. Therefore, we have studied total-DNA polymorphism from toxigenic and non toxigenic A. flavus strains isolated from maize crops and soil at two geographic locations, 300 km apart, in the Southeast region of Brazil. Total DNA from each A. flavus isolate was extracted and subjected to polymerase chain reaction amplification with five randomic primers through the RAPD (random amplified polymorphic DNA) technique. Phenetic and cladistic analyses of the data, based on bootstrap analyses, led us to conclude that RAPD was not suitable to discriminate toxigenic from non toxigenic strains. But the present results support the use of RAPD for strain characterization, especially for preliminary evaluation over extensive collections.-
Descrição: dc.descriptionInst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Metropolitanas Unit, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Santo Amaro, Fac Vet Med, Sao Paulo, Brazil-
Descrição: dc.descriptionInst Adolfo Lutz Registro, Sao Paulo, Brazil-
Descrição: dc.descriptionFdn Ezequiel Dias, Belo Horizonte, MG, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Metropolitanas Unit, Sao Paulo, Brazil-
Formato: dc.format687-692-
Idioma: dc.languageen-
Publicador: dc.publisherFundaco Oswaldo Cruz-
Relação: dc.relationMemorias Do Instituto Oswaldo Cruz-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectAspergillus flavus-
Palavras-chave: dc.subjectrandom amplified polymorphic DNA-
Palavras-chave: dc.subjectphylogeny genetic diversity-
Título: dc.titleGenetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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