Immunolocalization and effect of low concentrations of Insulin like growth factor-1 (IGF-1) in the canine ovary

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Autor(es): dc.contributorUniv Complutense Madrid-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorUniv Fed Para-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorMinist Agr Pecuaria & Abastecimento-
Autor(es): dc.creatorCardilli, Diogo J.-
Autor(es): dc.creatorSousa-Oliveira, Kellen-
Autor(es): dc.creatorFranchi-Joao, Carolina-
Autor(es): dc.creatorAzevedo-Voorwald, Faviana [UNESP]-
Autor(es): dc.creatorMachado-Silva, Marco A.-
Autor(es): dc.creatorOliveira, Joao Ademir [UNESP]-
Autor(es): dc.creatorSanchez-Calabuig, Maria Jesus-
Autor(es): dc.creatorToniollo, Gilson H. [UNESP]-
Autor(es): dc.creatorPerez-Gutierrez, Jose F.-
Data de aceite: dc.date.accessioned2022-02-22T00:11:47Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:47Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-09-07-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/vms3.347-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197283-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197283-
Descrição: dc.descriptionInsulin like growth factor-1 (IGF-1) plays an important role in the regulation of ovarian function. Despite its extensive study in several species, there is a paucity of information about IGF-1`s function and localization in the canine ovary. The aim of the present study was to assess the effect of IGF-1 on oocyte nuclear maturation and to immunolocalize the IGF-1 and its receptor (IGF-1R) in the ovary. Cumulus-oocyte complexes (COCs) were obtained from 34 bitches. The COCs from each bitch were incubated in TCM 199-HEPES in the absence (n = 199) or presence (n = 204) of 100 ng/ml IGF-1 for 96 hr at 38oC in 5% CO2, stained and evaluated for nuclear maturation by fluorescence microscopy. The results showed that the addition of IGF-1 did not have an effect (p > 0.05) on the nuclear maturation under these conditions. The immunohistochemical study revealed nuclear and cytoplasmic staining for IGF-1 and IGF-1R, respectively. Both were localized in all ovarian structures including the corpus luteum, but not in the granulosa cells from primordial follicles. In addition, IGF-1 was not localized in the oocytes in tertiary follicles. The results obtained show the presence of IGF-1 through the stages of follicular growth and in the corpus luteum of the canine ovary. However, its role on oocyte nuclear maturation could not be demonstrated.-
Descrição: dc.descriptionUniv Complutense Madrid, Fac Vet, Dept Med & Cirugia Anim, Av Puerta Hierro SN, Madrid 28040, Spain-
Descrição: dc.descriptionUniv Fed Goias, Escola Vet & Zootecnia, Dept Zootecnia, Goiania, Go, Brazil-
Descrição: dc.descriptionUniv Fed Para, Inst Med Vet, Castanhal, PA, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil-
Descrição: dc.descriptionUniv Fed Goias, Escola Vet & Zootecnia, Dept Med Vet, Goiania, Go, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias & Agr Vet, Dept Ciencias Exatas, Jaboticabal, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias & Agr Vet, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, Brazil-
Descrição: dc.descriptionMinist Agr Pecuaria & Abastecimento, Dept Inspecao Prod Origem Anim, Unidade Tecn Reg Uberlandia, Uberlandia, MG, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias & Agr Vet, Dept Ciencias Exatas, Jaboticabal, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias & Agr Vet, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, Brazil-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell-
Relação: dc.relationVeterinary Medicine And Science-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectbitch-
Palavras-chave: dc.subjectinsulin like growth factor-1-
Palavras-chave: dc.subjectmaturation-
Palavras-chave: dc.subjectoocyte-
Título: dc.titleImmunolocalization and effect of low concentrations of Insulin like growth factor-1 (IGF-1) in the canine ovary-
Tipo de arquivo: dc.typelivro digital-
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