Sodium caseinate improves longevity and fertility of frozen bull semen

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Fed Acre-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Vila Velha-
Autor(es): dc.creatorAlves Diniz, Jefferson Viana-
Autor(es): dc.creatorSatrapa, Rafael Augusto-
Autor(es): dc.creatorSegabinazzi, Lorenzo Garrido T. M. [UNESP]-
Autor(es): dc.creatorMatos Carneiro, Joao Alexandre [UNESP]-
Autor(es): dc.creatorOba, Eunice [UNESP]-
Autor(es): dc.creatorPapa, Federico Ozanam [UNESP]-
Autor(es): dc.creatorFreitas Dell'Aqua, Camila de Paula [UNESP]-
Autor(es): dc.creatorLoureiro, Barbara-
Autor(es): dc.creatorDell'Aqua Junior, Jose Antonio [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:11:42Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:42Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-09-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2020.05.028-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197251-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197251-
Descrição: dc.descriptionThis study aimed to evaluate the effect of sodium caseinate added into freezing extender on the sperm parameters of cryopreserved bull semen and in vitro and in vivo fertility. One ejaculate of 30 bulls was used and processed using Botu-Bov (Botupharma, Botucatu, Brazil) with the addition of 20% egg yolk (EY) or 15% egg yolk with 2% sodium caseinate (EY thorn SC), subsequently submitted to freezing. Semen from both groups were evaluated immediately after thawing (T0) and after thermic stress at 37 degrees C for 90 min (T90), for sperm kinetics, by CASA method, and plasma membrane integrity (PMI), superoxide (O-2) concentration and high mitochondrial potential (HMP) by flow cytometry. In vitro fertilization (IVF) was performed to assess embryo cleavage rate on day 3, and blastocyst rate on day 8. The in vivo fertility test was performed using fixed-time artificial insemination (FTAI). In sperm evaluation, trajectory velocity, linear velocity, curvilinear velocity, and lateral head movement were higher (P < 0.05) in EY thorn SC at T0. At T90, while rectilinearity and linearity did not differ between EY and EY thorn SC (P > 0.05), the other parameters evaluated were higher in EY thorn SC. Similarly, the integrity of the plasma and acrosomal membranes (iPAM) was higher (P < 0.05) at T90 in EY thorn SC, but did not differ (P > 0.05) between the groups at T0. For O-2(-) and HMP, the values were lower (P < 0.05) in EY thorn SC group in both moments; furthermore, EY thorn SC showed higher cleavage and blastocyst rates in IVF. Likewise, pregnancy rates by FTAI were higher (P < 0.05) in the EY thorn SC group. In conclusion, the addition of sodium caseinate into freezing extender improves sperm parameters of frozen-thawed bull semen and fertility rates on during in vitro and in vivo tests. (c) 2020 Elsevier Inc. All rights reserved.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Fed Acre, Programa Posgrad Sanidade & Prod Anim Sustentcive, Rodovia BR 364,Km 4, BR-69920900 Acre, AC, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, Rua Prof Doutor Walter Mauricio Correa S-N, BR-18618681 Sao Paulo, SP, Brazil-
Descrição: dc.descriptionUniv Vila Velha, Lab Reprod Physiol, Rua Viana S-N, BR-29102920 Vila Velha, ES, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, Rua Prof Doutor Walter Mauricio Correa S-N, BR-18618681 Sao Paulo, SP, Brazil-
Formato: dc.format59-65-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectBull semen-
Palavras-chave: dc.subjectFTAI-
Palavras-chave: dc.subjectFrozen semen-
Palavras-chave: dc.subjectCryopreservation-
Palavras-chave: dc.subjectIVP-
Título: dc.titleSodium caseinate improves longevity and fertility of frozen bull semen-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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