Novel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinantSaccharomyces cerevisiaestrains at high xylose concentrations

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Autor(es): dc.contributorBrazilian Ctr Res Energy & Mat CNPEM-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorRibeiro Bueno, Joao Gabriel-
Autor(es): dc.creatorBorelli, Guilherme-
Autor(es): dc.creatorRibeiro Correa, Thamy Livia-
Autor(es): dc.creatorFiamenghi, Mateus Bernabe-
Autor(es): dc.creatorJos, Juliana-
Autor(es): dc.creatorCarvalho, Murilo de-
Autor(es): dc.creatorOliveira, Leandro Cristante de [UNESP]-
Autor(es): dc.creatorPereira, Goncalo A. G.-
Autor(es): dc.creatorSantos, Leandro Vieira dos-
Data de aceite: dc.date.accessioned2022-02-22T00:11:40Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:40Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-08-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s13068-020-01782-0-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197237-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197237-
Descrição: dc.descriptionBackground The need to restructure the world's energy matrix based on fossil fuels and mitigate greenhouse gas emissions stimulated the development of new biobased technologies for renewable energy. One promising and cleaner alternative is the use of second-generation (2G) fuels, produced from lignocellulosic biomass sugars. A major challenge on 2G technologies establishment is the inefficient assimilation of the five-carbon sugar xylose by engineeredSaccharomyces cerevisiaestrains, increasing fermentation time. The uptake of xylose across the plasma membrane is a critical limiting step and the budding yeastS. cerevisiaeis not designed with a broad transport system and regulatory mechanisms to assimilate xylose in a wide range of concentrations present in 2G processes. Results Assessing diverse microbiomes such as the digestive tract of plague insects and several decayed lignocellulosic biomasses, we isolated several yeast species capable of using xylose. Comparative fermentations selected the yeastCandida sojaeas a potential source of high-affinity transporters. Comparative genomic analysis elects four potential xylose transporters whose properties were evaluated in the transporter null EBY.VW4000 strain carrying the xylose-utilizing pathway integrated into the genome. While the traditional xylose transporter Gxf1 allows an improved growth at lower concentrations (10 g/L), strains containing Cs3894 and Cs4130 show opposite responses with superior xylose uptake at higher concentrations (up to 50 g/L). Docking and normal mode analysis of Cs4130 and Gxf1 variants pointed out important residues related to xylose transport, identifying key differences regarding substrate translocation comparing both transporters. Conclusions Considering that xylose concentrations in second-generation hydrolysates can reach high values in several designed processes, Cs4130 is a promising novel candidate for xylose uptake. Here, we demonstrate a novel eukaryotic molecular transporter protein that improves growth at high xylose concentrations and can be used as a promising target towards engineering efficient pentose utilization in yeast.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionSerrapilheira Institute-
Descrição: dc.descriptionBrazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewable Natl Lab LNBR, BR-13083100 Campinas, SP, Brazil-
Descrição: dc.descriptionUniv Campinas UNICAMP, Inst Biol, Genet & Mol Biol Grad Program, Campinas, Brazil-
Descrição: dc.descriptionBrazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083970 Campinas, SP, Brazil-
Descrição: dc.descriptionBrazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, UNESP, Dept Phys, Inst Biosci Humanities & Exact Sci, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, UNESP, Dept Phys, Inst Biosci Humanities & Exact Sci, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2017/08519-6-
Descrição: dc.descriptionFAPESP: 2017/05078-9-
Descrição: dc.descriptionFAPESP: 2018/00888-5-
Descrição: dc.descriptionCNPq: 430291/2018-3-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionSerrapilheira Institute: Serra1708-16205-
Formato: dc.format20-
Idioma: dc.languageen-
Publicador: dc.publisherBmc-
Relação: dc.relationBiotechnology For Biofuels-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectXylose-
Palavras-chave: dc.subjectXylose transporter-
Palavras-chave: dc.subjectMajor facilitator superfamily-
Palavras-chave: dc.subjectSaccharomyces cerevisiae-
Palavras-chave: dc.subjectPentose metabolism-
Palavras-chave: dc.subjectIndustrial biotechnology-
Título: dc.titleNovel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinantSaccharomyces cerevisiaestrains at high xylose concentrations-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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