Karyotype changes in long-term cultured tick cell lines

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Autor(es): dc.contributorCzech Acad Sci-
Autor(es): dc.contributorUniv South Bohemia-
Autor(es): dc.contributorMasaryk Univ-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Castilla La Mancha-
Autor(es): dc.contributorUniv Liverpool-
Autor(es): dc.creatorKotsarenko, Kateryna-
Autor(es): dc.creatorVechtova, Pavlina-
Autor(es): dc.creatorLieskovska, Jaroslava-
Autor(es): dc.creatorFussy, Zoltan-
Autor(es): dc.creatorCabral-de-Mello, Diogo C. [UNESP]-
Autor(es): dc.creatorRego, Ryan O. M.-
Autor(es): dc.creatorAlberdi, Pilar-
Autor(es): dc.creatorCollins, Marisol-
Autor(es): dc.creatorBell-Sakyi, Lesley-
Autor(es): dc.creatorSterba, Jan-
Autor(es): dc.creatorGrubhoffer, Libor-
Data de aceite: dc.date.accessioned2022-02-22T00:11:33Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:33Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-08-10-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-020-70330-5-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197202-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197202-
Descrição: dc.descriptionTick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n=28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n=20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account.-
Descrição: dc.descriptionMinistry of Education, Youth and Sports of the Czech Republic INTER-ACTION project-
Descrição: dc.descriptionGrant Agency of the Czech Republic-
Descrição: dc.descriptionCzech research infrastructure for systems biology C4SYS-
Descrição: dc.descriptionUnited Kingdom Biotechnology and Biological Sciences Research Council-
Descrição: dc.descriptionMinistry of Education, Youth and Sports of the Czech Republic under the Projects CESNET-
Descrição: dc.descriptionCERIT-Scientific Cloud-
Descrição: dc.descriptionCzech Acad Sci, Inst Parasitol, Biol Ctr, Branisovska 31, Ceske Budejovice 37005, Czech Republic-
Descrição: dc.descriptionUniv South Bohemia, Fac Sci, Branisovska 1760, Ceske Budejovice 37005, Czech Republic-
Descrição: dc.descriptionMasaryk Univ, Cent European Inst Technol, Kamenice 5, Brno 62500, Czech Republic-
Descrição: dc.descriptionSao Paulo State Univ, Dept Gen & Appl Biol, Rio Claro, SP, Brazil-
Descrição: dc.descriptionUniv Castilla La Mancha, Reg Ctr Biomed Res CRIB, Ciudad Real Med Sch, Neuroplastic & Neurodegenerat Grp, Ciudad Real 13005, Spain-
Descrição: dc.descriptionUniv Liverpool, Inst Infect Vet & Ecol Sci, Dept Infect Biol & Microbiomes, Liverpool L3 5RF, Merseyside, England-
Descrição: dc.descriptionSao Paulo State Univ, Dept Gen & Appl Biol, Rio Claro, SP, Brazil-
Descrição: dc.descriptionMinistry of Education, Youth and Sports of the Czech Republic INTER-ACTION project: LTARF 18021-
Descrição: dc.descriptionGrant Agency of the Czech Republic: 15-03044S-
Descrição: dc.descriptionGrant Agency of the Czech Republic: 18-27204S-
Descrição: dc.descriptionCzech research infrastructure for systems biology C4SYS: LM2015055-
Descrição: dc.descriptionUnited Kingdom Biotechnology and Biological Sciences Research Council: BB/P024270/1-
Descrição: dc.descriptionMinistry of Education, Youth and Sports of the Czech Republic under the Projects CESNET: LM2015042-
Descrição: dc.descriptionCERIT-Scientific Cloud: LM2015085-
Formato: dc.format12-
Idioma: dc.languageen-
Publicador: dc.publisherNature Publishing Group-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleKaryotype changes in long-term cultured tick cell lines-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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