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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Czech Acad Sci | - |
Autor(es): dc.contributor | Univ South Bohemia | - |
Autor(es): dc.contributor | Masaryk Univ | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Univ Castilla La Mancha | - |
Autor(es): dc.contributor | Univ Liverpool | - |
Autor(es): dc.creator | Kotsarenko, Kateryna | - |
Autor(es): dc.creator | Vechtova, Pavlina | - |
Autor(es): dc.creator | Lieskovska, Jaroslava | - |
Autor(es): dc.creator | Fussy, Zoltan | - |
Autor(es): dc.creator | Cabral-de-Mello, Diogo C. [UNESP] | - |
Autor(es): dc.creator | Rego, Ryan O. M. | - |
Autor(es): dc.creator | Alberdi, Pilar | - |
Autor(es): dc.creator | Collins, Marisol | - |
Autor(es): dc.creator | Bell-Sakyi, Lesley | - |
Autor(es): dc.creator | Sterba, Jan | - |
Autor(es): dc.creator | Grubhoffer, Libor | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:11:33Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:11:33Z | - |
Data de envio: dc.date.issued | 2020-12-09 | - |
Data de envio: dc.date.issued | 2020-12-09 | - |
Data de envio: dc.date.issued | 2020-08-10 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1038/s41598-020-70330-5 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/197202 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/197202 | - |
Descrição: dc.description | Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n=28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n=20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account. | - |
Descrição: dc.description | Ministry of Education, Youth and Sports of the Czech Republic INTER-ACTION project | - |
Descrição: dc.description | Grant Agency of the Czech Republic | - |
Descrição: dc.description | Czech research infrastructure for systems biology C4SYS | - |
Descrição: dc.description | United Kingdom Biotechnology and Biological Sciences Research Council | - |
Descrição: dc.description | Ministry of Education, Youth and Sports of the Czech Republic under the Projects CESNET | - |
Descrição: dc.description | CERIT-Scientific Cloud | - |
Descrição: dc.description | Czech Acad Sci, Inst Parasitol, Biol Ctr, Branisovska 31, Ceske Budejovice 37005, Czech Republic | - |
Descrição: dc.description | Univ South Bohemia, Fac Sci, Branisovska 1760, Ceske Budejovice 37005, Czech Republic | - |
Descrição: dc.description | Masaryk Univ, Cent European Inst Technol, Kamenice 5, Brno 62500, Czech Republic | - |
Descrição: dc.description | Sao Paulo State Univ, Dept Gen & Appl Biol, Rio Claro, SP, Brazil | - |
Descrição: dc.description | Univ Castilla La Mancha, Reg Ctr Biomed Res CRIB, Ciudad Real Med Sch, Neuroplastic & Neurodegenerat Grp, Ciudad Real 13005, Spain | - |
Descrição: dc.description | Univ Liverpool, Inst Infect Vet & Ecol Sci, Dept Infect Biol & Microbiomes, Liverpool L3 5RF, Merseyside, England | - |
Descrição: dc.description | Sao Paulo State Univ, Dept Gen & Appl Biol, Rio Claro, SP, Brazil | - |
Descrição: dc.description | Ministry of Education, Youth and Sports of the Czech Republic INTER-ACTION project: LTARF 18021 | - |
Descrição: dc.description | Grant Agency of the Czech Republic: 15-03044S | - |
Descrição: dc.description | Grant Agency of the Czech Republic: 18-27204S | - |
Descrição: dc.description | Czech research infrastructure for systems biology C4SYS: LM2015055 | - |
Descrição: dc.description | United Kingdom Biotechnology and Biological Sciences Research Council: BB/P024270/1 | - |
Descrição: dc.description | Ministry of Education, Youth and Sports of the Czech Republic under the Projects CESNET: LM2015042 | - |
Descrição: dc.description | CERIT-Scientific Cloud: LM2015085 | - |
Formato: dc.format | 12 | - |
Idioma: dc.language | en | - |
Publicador: dc.publisher | Nature Publishing Group | - |
Relação: dc.relation | Scientific Reports | - |
???dc.source???: dc.source | Web of Science | - |
Título: dc.title | Karyotype changes in long-term cultured tick cell lines | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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