Correlation between cervical HPV DNA detection and HPV16 seroreactivity measured with L1-only and L1+L2 viral capsid antigens

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Montreal-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorLudwig Inst Canc Res-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorMcGill Univ-
Autor(es): dc.creatorTrevisan, Andrea-
Autor(es): dc.creatorCandeias, Joao M. G. [UNESP]-
Autor(es): dc.creatorThomann, Patricia-
Autor(es): dc.creatorVilla, Luisa L.-
Autor(es): dc.creatorFranco, Eduardo L.-
Autor(es): dc.creatorTrottier, Helen-
Autor(es): dc.creatorLudwig McGill Study Grp-
Data de aceite: dc.date.accessioned2022-02-22T00:11:32Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:32Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1099/jmm.0.001213-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197197-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197197-
Descrição: dc.descriptionIntroduction. Persistent human papillomavirus (HPV) type 16 infection is the main causal agent of cervical cancer. Most HPV infections clear spontaneously within 1-2 years. Although not all infected women develop detectable HPV antibodies, about 60-70% seroconvert and retain their antibodies at low levels. Aim. We investigated if cervical HPV16 DNA positivity was associated with HPV16 seroreactivity measured with two different antigen formulations. We assessed if associations were influenced by co-infection with other HPV types and HPV16 viral load. Methodology. We used baseline data for women participating in the Ludwig-McGill cohort, a longitudinal investigation of the natural history of HPV infection and cervical neoplasia. The study enrolled 2462 Brazilian women from 1993 to 1997 (pre-vaccination). ELISA assays were based on L1-only or L1+L2 virus-like particles (VLPs). Seroreactivity was expressed as normalized absorbance ratios. HPV genotyping and viral load were evaluated by PCR protocols. Pearson's r was used to measure correlations between interval-scaled variables. Serological accuracy in HPV16 DNA detection was assessed using receiver operating characteristic (ROC) curves. We analysed the association between HPV DNA positivity and HPV16 seroreactivity by linear regression. Results. Correlations between L1+L2 and L1-only VLPs for detection of HPV16 were poor (r=0.43 and 0.44 for dilutions 1 :10 and 1:50, respectively). The protocol with the best accuracy was L1+L2 VLPs at serum dilution 1: 10 (ROC area=0.73, 95% CI: 0.65-0.85). HPV16 DNA positivity was correlated with HPV16 seroreactivity and was not influenced by co-infection or viral load. To a lesser degree, HPV16 seroreactivity was correlated with infection by other Alpha-9 papillomavirus species. Conclusion. HPV16 DNA positivity and HPV16 seroreactivity are strongly correlated. L1+L2 VLPs perform better than L1-only VLPs for detecting IgG antibodies to HPV16 in women infected with HPV16 or other Alpha-9 HPV species. This study advances our understanding of humoral immune responses against HPV16 by providing insights about the influence of VLP antigen composition to measure humoral immune response against naturally acquired HPV infection.-
Descrição: dc.descriptionLudwig Institute for Cancer Research-
Descrição: dc.descriptionU.S. National Cancer Institute-
Descrição: dc.descriptionCanadian Institutes of Health Research-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCanadian Institutes of Health Research [BESC--M]-
Descrição: dc.descriptionFond de la recherche en sante du Quebec-
Descrição: dc.descriptionUniv Montreal, Sch Publ Hlth, Dept Social & Prevent Med, Montreal, PQ, Canada-
Descrição: dc.descriptionUniv Montreal, St Justine Hosp Res Ctr, Montreal, PQ, Canada-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP, Brazil-
Descrição: dc.descriptionLudwig Inst Canc Res, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Inst Canc Estado Sao Paulo, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Med, Dept Radiol & Oncol, Sao Paulo, Brazil-
Descrição: dc.descriptionMcGill Univ, Div Canc Epidemiol, Montreal, PQ, Canada-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP, Brazil-
Descrição: dc.descriptionU.S. National Cancer Institute: CA70269-
Descrição: dc.descriptionCanadian Institutes of Health Research: MOP-49396-
Descrição: dc.descriptionCanadian Institutes of Health Research: CRN-83320-
Descrição: dc.descriptionFAPESP: 1999/10790-0-
Descrição: dc.descriptionFAPESP: 1994/05415-1-
Descrição: dc.descriptionFAPESP: 1997/07172-7-
Formato: dc.format960-970-
Idioma: dc.languageen-
Publicador: dc.publisherMicrobiology Soc-
Relação: dc.relationJournal Of Medical Microbiology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectELISA-
Palavras-chave: dc.subjectHuman papillomavirus-
Palavras-chave: dc.subjectHPV16 seroreactivity-
Palavras-chave: dc.subjectIgG antibodies-
Palavras-chave: dc.subjectnatural infection-
Palavras-chave: dc.subjectvirus-like particles-
Título: dc.titleCorrelation between cervical HPV DNA detection and HPV16 seroreactivity measured with L1-only and L1+L2 viral capsid antigens-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.