Sugarcane cystatin CaneCPI-1 promotes osteogenic differentiation in human dental pulp cells: a new insight into cysteine proteases inhibitors

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorRodrigues, E. M. [UNESP]-
Autor(es): dc.creatorViola, K. S. [UNESP]-
Autor(es): dc.creatorGomes-Cornelio, A. L. [UNESP]-
Autor(es): dc.creatorSoares-Costa, A.-
Autor(es): dc.creatorHenrique-Silva, F.-
Autor(es): dc.creatorRossa-Junior, C. [UNESP]-
Autor(es): dc.creatorGuerreiro-Tanomaru, J. M. [UNESP]-
Autor(es): dc.creatorTanomaru-Filho, M. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:11:32Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:32Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-08-18-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/iej.13371-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197195-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197195-
Descrição: dc.descriptionAim To investigate the biocompatibility, type of cell death, osteogenic bioactivity and mRNA expression of the osteogenic markers, induced by CaneCPI-1 in human dental pulp cells (hDPCs). Methodology hDPCs exposed to CaneCPI-1 and not exposed (control) were evaluated for cell viability by the 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay; apoptosis by flow cytometry; alkaline phosphatase (ALP) activity by calculation of thymolphthalein release; gene expression of bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX2), ALP, osteocalcin (OC), bone sialoprotein (BSP) by qPCR; and mineralized nodules production by using alizarin red staining. The data were analysed by one-way analysis of variance (anova) and Turkey's post-test, two-wayanovaand Bonferroni post-test or t-test (P < 0.05). Results CaneCPI-1 induced no apoptosis and had no cytotoxic effect, except in the concentration of 33.20 mu m, in which cell viability was significantly lower than the control (alpha-MEM nonosteogenic medium serum-free) (P < 0.05). There was significantly greater ALP activity, greater expression of the BMP-2, RUNX2, ALP, OC and BSP genes and greater mineralized nodules production in the CaneCPI-1 group in comparison with the control or osteogenic alpha-MEM control (alpha-MEM osteogenic medium - L-ascorbic acid and beta-glycerophosphate) (P < 0.05). Conclusions CaneCPI-1 was cytocompatible and also induced the differentiation of hDPCs in osteogenic phenotypein vitro. CaneCPI-1 is a promising molecule to induce pulp repair.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSao Paulo State Univ, Dept Restorat Dent, Dent Sch, Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, Brazil-
Descrição: dc.descriptionDent Sch Sao Paulo State Univ, Dept Diag & Surg, Araraquara, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Restorat Dent, Dent Sch, Araraquara, SP, Brazil-
Descrição: dc.descriptionDent Sch Sao Paulo State Univ, Dept Diag & Surg, Araraquara, SP, Brazil-
Descrição: dc.descriptionCAPES: 2019/88887.351608/2019-00-
Descrição: dc.descriptionFAPESP: 2017/19049-0-
Formato: dc.format9-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell-
Relação: dc.relationInternational Endodontic Journal-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectcanecystatin-
Palavras-chave: dc.subjectcell differentiation-
Palavras-chave: dc.subjectcysteine proteinase inhibitor-
Palavras-chave: dc.subjecthuman dental pulp cells-
Título: dc.titleSugarcane cystatin CaneCPI-1 promotes osteogenic differentiation in human dental pulp cells: a new insight into cysteine proteases inhibitors-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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