Flow cytometric analysis from fish samples stored at low, ultra-low and cryogenic temperatures

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorChico Mendes Inst Biodivers Conservat-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorAES Tiete-
Autor(es): dc.creatorYasui, George Shigueki-
Autor(es): dc.creatorBertolini, Rafaela Manchin [UNESP]-
Autor(es): dc.creatorSuarez-Lopez, Lucia [UNESP]-
Autor(es): dc.creatorXavier, Pedro Porfirio-
Autor(es): dc.creatorMonzani, Paulo Sergio-
Autor(es): dc.creatorNascimento, Nivaldo Ferreira do-
Autor(es): dc.creatorCastilho, Antonio Leao [UNESP]-
Autor(es): dc.creatorOkada Nakaghi, Laura Satiko [UNESP]-
Autor(es): dc.creatorAlves dos Santos, Silvio Carlos-
Autor(es): dc.creatorSenhorini, Jose Augusto-
Data de aceite: dc.date.accessioned2022-02-22T00:11:26Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:26Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.cryobiol.2020.06.004-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197152-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197152-
Descrição: dc.descriptionFlow cytometry is a valuable tool in biomedical and animal sciences. However, equipment used for such analysis presents limitations at field conditions, suggesting then preservation procedures for future analysis at laboratory conditions. In this study, freezing at low (-20 degrees C), ultra-low (-80 degrees C) and cryogenic temperatures (-196 degrees C, i.e. liquid nitrogen) were used as preservation procedures of fish tissue. Samples were maintained in 0.9% NaCl or lysing solution, and stored at the temperatures above for 0 (fresh control), 60, 120 and 180 days of storage. After storage, the samples were thawed and proceeded to flow cytometric analysis. Storage at low temperatures (-20 degrees C), both in lysing and 0.9% NaCl, exhibited poor results when analyzed after 60, 120 and 180 days, showing noisy peaks, deviation in the DNA content and absence of peaks. Ultralow (-80 degrees C) and cryogenic (-196 degrees C) temperatures, both in lysing solution and 0.9% NaCl, showed good results and high quality of histograms. Both storage procedures gave similar histograms and DNA content in comparison with control group (fresh) even after 60, 120 and 180 days of storage, exhibiting the main peak at 2C content from diploid cells and a secondary peak at 4C derived from dividing cells. In conclusion, samples may be stored for 180 days at -80 degrees C and -196 degrees C in both, 0.9% NaCl or lysing solution. As cryogenic temperatures in liquid nitrogen permits indefinite storage, this procedure should be used for long-term preservation.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionAES Tiete (Research & Development Program ANEEL)-
Descrição: dc.descriptionUniv Sao Paulo, Dept Anim Reprod FMVZ, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil-
Descrição: dc.descriptionChico Mendes Inst Biodivers Conservat, Lab Fish Biotechnol, Natl Ctr Res & Conservat Continental Fish, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ Julio de Mesquita Filho, Inst Biosci, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Dept Vet Med FZEA, Ave Duque Caxias Norte 225, BR-13630080 Pirassununga, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil-
Descrição: dc.descriptionAES Tiete, Br-153,0 Km 139 Ctr, BR-16370000 Promissao, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ Julio de Mesquita Filho, Inst Biosci, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2010/17429-1-
Descrição: dc.descriptionFAPESP: 2011/11664-1-
Descrição: dc.descriptionAES Tiete (Research & Development Program ANEEL): 0064-1042/2014-
Formato: dc.format68-71-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationCryobiology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectCell cycle-
Palavras-chave: dc.subjectDNA quantity-
Palavras-chave: dc.subjectFish-
Palavras-chave: dc.subjectPloidy-
Palavras-chave: dc.subjectStorage-
Título: dc.titleFlow cytometric analysis from fish samples stored at low, ultra-low and cryogenic temperatures-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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