What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA?

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorJackson Lab-
Autor(es): dc.creatorColombini-Ishikiriama, Bella Luna-
Autor(es): dc.creatorDionisio, Thiago Jose-
Autor(es): dc.creatorGarbieri, Thais Francini-
Autor(es): dc.creatorSilva, Rafaela Alves da-
Autor(es): dc.creatorAndrade Moreira Machado, Maria Aparecida-
Autor(es): dc.creatorPenha de Oliveira, Sandra Helena [UNESP]-
Autor(es): dc.creatorLara, Vanessa Soares-
Autor(es): dc.creatorGreene, Andrew Seth-
Autor(es): dc.creatorSantos, Carlos Ferreira-
Data de aceite: dc.date.accessioned2022-02-22T00:11:03Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:03Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-06-22-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s12865-020-00367-8-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197014-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197014-
Descrição: dc.descriptionBackground Oral fibroblast immunological responses to bacterial stimuli are well known. However, there are few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which are important for the treatment of pulp infections in children. The aim of this study was to evaluate expression and production of inflammatory cytokines and chemokines by HDPF when challenged with bacterial antigens normally present in advanced caries lesions. Methods Triplicate HDPF from 4 children (n = 4; 2 boys and 2 girls) were cultured by explant technique and challenged or not withEscherichia colilipopolysaccharide/1 mu g/mL(EcLPS)orEnterococcus faecalislipoteichoic acid/1 mu g/mL(EfLTA)for 6 and 24 h. Most of published studies employed immortalized cells, i.e., without checking possible gender and genetic variables. mRNA expression and protein production were evaluated by RT-qPCR and ELISA MILLIPLEX (R), respectively, for Interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, Chemokine C-C motif ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1), Chemokine C-C motif ligand 3/macrophage inflammatory protein 1-alpha (CCL3/MIP1-alpha), Chemokine C-C motif ligand 5/ regulated on activation, normal T cell expressed and secreted (CCL5/RANTES), C-X-C motif chemokine 12/ stromal cell-derived factor 1 (CXCL12/SDF-1), Tumor Necrosis Factor-alpha (TNF-alpha), Interferon-gamma (IFN gamma), Vascular Endothelial Growth Factor (VEGF), Colony stimulating factor 1 (CSF-1) and Macrophage colony-stimulating factor (M-CSF). Results EcLPSincreased IL-1 alpha, IL-1 beta, IL-8, CCL2, CCL5, TNF-alpha and CSF-1 mRNA and protein levels whileEfLTAwas only able to positively regulate gene expression and protein production of IL-8. Conclusion The results of the present study confirmed our hypothesis, since pulp fibroblasts from deciduous teeth are capable of increasing gene expression and protein production after being stimulated withEcLPSandEfLTA.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, Bauru, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Dent Aracatuba, Dept Basic Sci, Aracatuba, SP, Brazil-
Descrição: dc.descriptionJackson Lab, 600 Main St, Bar Harbor, ME 04609 USA-
Descrição: dc.descriptionSao Paulo State Univ, Sch Dent Aracatuba, Dept Basic Sci, Aracatuba, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2015/03965-2-
Descrição: dc.descriptionFAPESP: 2016/11450-5-
Descrição: dc.descriptionCNPq: 307986/2017-9-
Descrição: dc.descriptionCAPES: 001-
Formato: dc.format9-
Idioma: dc.languageen-
Publicador: dc.publisherBmc-
Relação: dc.relationBmc Immunology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectCytokines-
Palavras-chave: dc.subjectDental pulp-
Palavras-chave: dc.subjectFibroblasts and lipopolysaccharide-
Título: dc.titleWhat is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA?-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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