Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria

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Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Estado Santa Catarina-
Autor(es): dc.creatorPerez Navarro, Miguel Octavio-
Autor(es): dc.creatorDilarri, Guilherme [UNESP]-
Autor(es): dc.creatorSimionato, Ane Stefano-
Autor(es): dc.creatorGrzegorczyk, Kathlen-
Autor(es): dc.creatorDealis, Mickely Liuti-
Autor(es): dc.creatorCano, Barbara Gionco-
Autor(es): dc.creatorBarazetti, Andre Riedi-
Autor(es): dc.creatorAfonso, Leandro-
Autor(es): dc.creatorChryssafidis, Andreas Lazaros-
Autor(es): dc.creatorFerreira, Henrique [UNESP]-
Autor(es): dc.creatorAndrade, Galdino-
Data de aceite: dc.date.accessioned2022-02-22T00:11:03Z-
Data de disponibilização: dc.date.available2022-02-22T00:11:03Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-06-04-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fmicb.2020.01076-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197012-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197012-
Descrição: dc.descriptionThe antibiotic activity of metalloantibiotic compounds has been evaluated since the 90s, and many different modes of action were characterized. In the last decade, the effects of secondary metabolites produced byPseudomonas aeruginosaLV strain, including a cupric compound identified as Fluopsin C, were tested against many pathogenic bacteria strains, proving their high antibiotic activity. In the present study, the bactericidal mechanisms of action of Fluopsin C and the semi-purified fraction F4A were elucidated. The results found in electron microscopy [scanning electron microscopy (SEM) and transmission electronic microscopy (TEM)] demonstrated that both Fluopsin C and F4A are affecting the cytoplasmatic membrane of Gram-positive and Gram-negative bacteria. These results were confirmed by fluorescence microscopy, where these bacteria presented permeabilization of their cytoplasmatic membranes after contact with the semi-purified fraction and pure compound. Using electronic and fluorescence microscopy, along with bacterial mutant strains with marked divisional septum, the membrane was defined as the primary target of Fluopsin C in the tested bacteria.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionAraucaria Foundation-
Descrição: dc.descriptionUniv Estadual Londrina, Dept Microbiol, Microbial Ecol Lab, Londrina, Parana, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biosci, Dept Biochem & Microbiol, Rio Claro, Brazil-
Descrição: dc.descriptionUniv Estado Santa Catarina, Ctr Agrovet Sci, Dept Vet Med, Lages, SC, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biosci, Dept Biochem & Microbiol, Rio Claro, Brazil-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherFrontiers Media Sa-
Relação: dc.relationFrontiers In Microbiology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectbactericidal mechanism of action-
Palavras-chave: dc.subjectXanthomonas-
Palavras-chave: dc.subjectKPC-
Palavras-chave: dc.subjectMRSA-
Palavras-chave: dc.subjectelectronic microscopy-
Palavras-chave: dc.subjectfluorescence microscopy-
Título: dc.titleDetermining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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