The nuclear and mitochondrial genomes of Frieseomelitta varia - a highly eusocial stingless bee (Meliponini) with a permanently sterile worker caste

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniv Fed Alfenas-
Autor(es): dc.contributorUniversidade Federal de Viçosa (UFV)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniv Tecnol Fed Parana-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorPaula Freitas, Flavia C. de-
Autor(es): dc.creatorLourenco, Anete P.-
Autor(es): dc.creatorNunes, Francis M. F.-
Autor(es): dc.creatorPaschoal, Alexandre R.-
Autor(es): dc.creatorAbreu, Fabiano C. P.-
Autor(es): dc.creatorBarbin, Fabio O.-
Autor(es): dc.creatorBataglia, Luana-
Autor(es): dc.creatorCardoso-Junior, Carlos A. M.-
Autor(es): dc.creatorCervoni, Mario S.-
Autor(es): dc.creatorSilva, Saura R. [UNESP]-
Autor(es): dc.creatorDalarmi, Fernanda-
Autor(es): dc.creatorDel Lama, Marco A.-
Autor(es): dc.creatorDepintor, Thiago S.-
Autor(es): dc.creatorFerreira, Katia M.-
Autor(es): dc.creatorGoria, Paula S.-
Autor(es): dc.creatorJaskot, Michael C.-
Autor(es): dc.creatorLago, Denyse C.-
Autor(es): dc.creatorLuna-Lucena, Danielle-
Autor(es): dc.creatorModa, Livia M.-
Autor(es): dc.creatorNascimento, Leonardo-
Autor(es): dc.creatorPedrino, Matheus-
Autor(es): dc.creatorOliveira, Franciene Rabico-
Autor(es): dc.creatorSanches, Fernanda C.-
Autor(es): dc.creatorSantos, Douglas E.-
Autor(es): dc.creatorSantos, Carolina G.-
Autor(es): dc.creatorVieira, Joseana-
Autor(es): dc.creatorBarchuk, Angel R.-
Autor(es): dc.creatorHartfelder, Klaus-
Autor(es): dc.creatorSimoes, Zila L. P.-
Autor(es): dc.creatorBitondi, Marcia M. G.-
Autor(es): dc.creatorPinheiro, Daniel G. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:10:58Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:58Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-06-03-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s12864-020-06784-8-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196980-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196980-
Descrição: dc.descriptionBackgroundMost of our understanding on the social behavior and genomics of bees and other social insects is centered on the Western honey bee, Apis mellifera. The genus Apis, however, is a highly derived branch comprising less than a dozen species, four of which genomically characterized. In contrast, for the equally highly eusocial, yet taxonomically and biologically more diverse Meliponini, a full genome sequence was so far available for a single Melipona species only. We present here the genome sequence of Frieseomelitta varia, a stingless bee that has, as a peculiarity, a completely sterile worker caste.ResultsThe assembly of 243,974,526 high quality Illumina reads resulted in a predicted assembled genome size of 275Mb composed of 2173 scaffolds. A BUSCO analysis for the 10,526 predicted genes showed that these represent 96.6% of the expected hymenopteran orthologs. We also predicted 169,371 repetitive genomic components, 2083 putative transposable elements, and 1946 genes for non-coding RNAs, largely long non-coding RNAs. The mitochondrial genome comprises 15,144bp, encoding 13 proteins, 22 tRNAs and 2 rRNAs. We observed considerable rearrangement in the mitochondrial gene order compared to other bees. For an in-depth analysis of genes related to social biology, we manually checked the annotations for 533 automatically predicted gene models, including 127 genes related to reproductive processes, 104 to development, and 174 immunity-related genes. We also performed specific searches for genes containing transcription factor domains and genes related to neurogenesis and chemosensory communication.ConclusionsThe total genome size for F. varia is similar to the sequenced genomes of other bees. Using specific prediction methods, we identified a large number of repetitive genome components and long non-coding RNAs, which could provide the molecular basis for gene regulatory plasticity, including worker reproduction. The remarkable reshuffling in gene order in the mitochondrial genome suggests that stingless bees may be a hotspot for mtDNA evolution. Hence, while being just the second stingless bee genome sequenced, we expect that subsequent targeting of a selected set of species from this diverse clade of highly eusocial bees will reveal relevant evolutionary signals and trends related to eusociality in these important pollinators.-
Descrição: dc.descriptionBrazilian National Science Fund (Conselho Nacional de Desenvolvimento Cientifico and Tecnologico)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionUniv Fed Alfenas, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Alfenas, MG, Brazil-
Descrição: dc.descriptionUniv Fed Vales Jequitinhonha & Mucuri, Fac Ciencias Biol & Saude, Dept Ciencias Biol, Diamantina, MG, Brazil-
Descrição: dc.descriptionUniv Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, Sao Carlos, SP, Brazil-
Descrição: dc.descriptionUniv Tecnol Fed Parana, Cornelio Procopio, PR, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionUniv, Estadual Paulista Julio de Mesquita Filho, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionUniv, Estadual Paulista Julio de Mesquita Filho, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil-
Descrição: dc.descriptionBrazilian National Science Fund (Conselho Nacional de Desenvolvimento Cientifico and Tecnologico): 454103/2014-0-
Descrição: dc.descriptionFAPESP: 2015/06657-0-
Descrição: dc.descriptionCAPES: 001-
Formato: dc.format26-
Idioma: dc.languageen-
Publicador: dc.publisherBmc-
Relação: dc.relationBmc Genomics-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectSocial insect-
Palavras-chave: dc.subjectMeliponini-
Palavras-chave: dc.subjectIllumina sequencing-
Palavras-chave: dc.subjectGenome assembly-
Palavras-chave: dc.subjectSynteny-
Palavras-chave: dc.subjectRepetitive elements-
Palavras-chave: dc.subjectNon-coding RNA-
Palavras-chave: dc.subjectReproductive process genes-
Palavras-chave: dc.subjectImmunity genes-
Título: dc.titleThe nuclear and mitochondrial genomes of Frieseomelitta varia - a highly eusocial stingless bee (Meliponini) with a permanently sterile worker caste-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.