Characterization and strong risk association of TLR2 del -196 to -174 polymorphism and Helicobacter pylori and their influence on mRNA expression in gastric cancer

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Sagrado Coracao-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorLourenco, Caroline de Matos-
Autor(es): dc.creatorSusi, Manoela Dias-
Autor(es): dc.creatorAntunes do Nascimento, Mariah Cristina-
Autor(es): dc.creatorSerafim Junior, Vilson-
Autor(es): dc.creatorSimedan Vila, Ana Paula-
Autor(es): dc.creatorRodrigues-Flemming, Gabriela Helena-
Autor(es): dc.creatorGoloni-Bertollo, Eny Maria-
Autor(es): dc.creatorSilva, Ana Elizabete [UNESP]-
Autor(es): dc.creatorOliveira-Cucolo, Juliana Garcia de-
Data de aceite: dc.date.accessioned2022-02-22T00:10:49Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:49Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-05-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.4251/wjgo.v12.i5.535-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196927-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196927-
Descrição: dc.descriptionBACKGROUND Toll-like receptor-2 (TLR2) is responsible for recognizing Helicobacter pylori (H. pylori) and activating the immune response. Polymorphisms in TLR2 may modulate gastric carcinogenesis. AIM To evaluate whether the TLR2 19216T/C (rs3804099) and TLR2 -196 to -174 ins/del (rs111200466) polymorphisms contribute to gastric carcinogenesis in the Brazilian population, and to determine the influence of both polymorphisms and H. pylori infection on TLR2 mRNA expression. METHODS DNA was extracted from 854 peripheral blood leukocyte or gastric tissue samples [202 gastric cancer (GC), 269 chronic gastritis (CG), and 383 control/healthy (C)] and genotyped by allele-specific PCR or restriction fragment length polymorphism (RFLP)-PCR. Quantitative polymerase chain reaction by TaqMan(R) assay was used to quantify TLR2 mRNA levels in fresh gastric tissues (48 GC, 36 CG, and 14 C). RESULTS Regarding the TLR2 -196 to -174 polymorphism, the ins/del and del/del genotypes were associated with a higher risk of GC by comparison with the C in all of the analyzed inheritance models (codominant, dominant, recessive, overdominant and log-additive; P < 0.0001). Similarly, an increased risk was observed when comparing the GC and CG groups [codominant (P < 0.0001), dominant (P < 0.0001), recessive (P = 0.0260), overdominant (P < 0.0001) and log-additive (P < 0.0001)]. In contrast, TLR2 19216T/C was associated with a protective effect in the GC group compared to the C group [dominant (P = 0.0420) and log-additive (P = 0.0300)]. Regarding the association of polymorphisms with H. pylori infection, individuals infected with H. pylori and harboring the TLR2 -196 to -174 ins/del polymorphism had an increased risk of gastric carcinogenesis [codominant (P = 0.0120), dominant (P = 0.0051), overdominant (P = 0.0240) and log-additive (P = 0.0030)], while TLR2 19216T/C was associated with a protective effect [codominant (P = 0.0039), dominant (P < 0.0001), overdominant (P = 0.0097) and log-additive (P = 0.0021)]. TLR2 mRNA levels were significantly increased in the GC group (median RQ = 6.95) compared to the CG group (RQ = 0.84, P < 0.0001) and to the normal mucosa group (RQ = 1.0). In addition, both H. pylori infection (P < 0.0001) and the presence of the polymorphic TLR2 -196 to -174del (P = 0.0010) and TLR2 19216 C (P = 0.0004) alleles influenced TLR2 mRNA expression. CONCLUSION The TLR2 -196 to -174 ins/del and TLR2 19216 T/C polymorphisms are strongly associated with GC. TLR2 mRNA expression levels are upregulated in neoplastic tissues and influenced by both the presence of H. pylori and variant genotypes.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Sagrado Coracao, Dept Grad Level Res, BR-17011970 Bauru, SP, Brazil-
Descrição: dc.descriptionSao Jose do Rio Preto Sch Med, Dept Mol Biol, BR-15090000 Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Biol, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Biol, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2013/14022-6-
Descrição: dc.descriptionFAPESP: 2014/177161-
Formato: dc.format535-548-
Idioma: dc.languageen-
Publicador: dc.publisherBaishideng Publishing Group Inc-
Relação: dc.relationWorld Journal Of Gastrointestinal Oncology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectToll-like receptor 2-
Palavras-chave: dc.subjectHelicobacter pylori-
Palavras-chave: dc.subjectGastric cancer-
Palavras-chave: dc.subjectChronic gastritis-
Palavras-chave: dc.subjectPolymorphisms-
Palavras-chave: dc.subjectGene expression-
Título: dc.titleCharacterization and strong risk association of TLR2 del -196 to -174 polymorphism and Helicobacter pylori and their influence on mRNA expression in gastric cancer-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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