Performance of rK39-based immunochromatographic rapid diagnostic test for serodiagnosis of visceral leishmaniasis using whole blood, serum and oral fluid

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorInst Infectol Emilio Ribas-
Autor(es): dc.contributorUniversidade Federal de Sergipe (UFS)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Mato Grosso do Sul (UFMS)-
Autor(es): dc.contributorUniv Fed Rio Grande do Norte-
Autor(es): dc.contributorHosp Giselda Trigueiro-
Autor(es): dc.creatorArroyo Sanchez, Maria Carmen-
Autor(es): dc.creatorCeleste, Beatriz Julieta-
Autor(es): dc.creatorLauletta Lindoso, Jose Angelo-
Autor(es): dc.creatorFujimori, Mahyumi-
Autor(es): dc.creatorAlmeida, Roque Pacheco de-
Autor(es): dc.creatorCastelo Branco Fortaleza, Carlos Magno [UNESP]-
Autor(es): dc.creatorDruzian, Angelita Fernandes-
Autor(es): dc.creatorFreitas Lemos, Ana Priscila-
Autor(es): dc.creatorAndrade de Melo, Vanessa Campos-
Autor(es): dc.creatorMiranda Paniago, Anamaria Mello-
Autor(es): dc.creatorQueiroz, Igor Thiago-
Autor(es): dc.creatorGoto, Hiro-
Data de aceite: dc.date.accessioned2022-02-22T00:10:48Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:48Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-04-02-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0230610-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196919-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196919-
Descrição: dc.descriptionBackground The development of rK39-based immunochromatographic rapid diagnostic tests represents an important advance for serodiagnosis of visceral leishmaniasis, being cheap and easy to use at the point of care (POC). Although the use of rK39 have considerably improved the sensitivity and specificity of serological tests compared with total antigens, great variability in sensitivity and specificity was reported. This study aimed at the evaluation of Kalazar Detect (TM) Rapid Test, Whole Blood (Kalazar Detect RDT) for Visceral Leishmaniasis (VL) diagnosis using oral fluid, whole blood and serum specimens collected at different endemic areas of VL of Brazil. Methodology To evaluate Kalazar Detect RDT, oral fluid, whole blood and serum specimens from 128 VL patients, 85 healthy individuals, 22 patients with possible cross-reactivity diseases and 20 VL/aids coinfected patients were collected and assayed at the POC. Principal findings and conclusions The performance of Kalazar Detect RDT in whole blood and serum was similar; however, using oral fluid, the sensitivity was low. Particularly in samples from the city of Natal, Rio Grande do Norte state in Northeastern Brazil, we observed low sensitivity, 80.0% (95% CI: 62.7-90.5), using whole blood and serum, and poor sensitivity, 43.3% (95% CI: 27.4-60.8) with oral fluid. Those values were much lower than in the other regions, where sensitivity ranged from 92.7-96.3% in whole blood and serum, and 80.0-88.9% in oral fluid. Besides, in VL/aids coinfected patients, lower sensitivity was achieved compared with VL patients. In samples from Natal, the sensitivity was 0.0% (95% CI: 0.0-49.0) and 25.0% (95% CI: 4.6-69.9), using oral fluid and serum/whole blood, respectively; in samples from the other regions, the sensitivity ranged from 40.0-63.6% and 80.0-81.8%, respectively. As for specificity, high values were observed across the fluids, 100.0% (95% CI: 96.5-100.0) in whole blood, 96.3% (95% CI: 90.8-98.5) in serum, and 95.3% (95% CI: 89.5-98.0) in oral fluid; across localities, specificity ranged from 85.7-100.0%. Serum samples sent by the collaborating centers to Instituto de Medicina Tropical (n = 250) were tested by Kalazar Detect RDT, Direct Agglutination Test, Indirect immunofluorescence assay, Enzyme-linked immunosorbent assay, and IT-Leish (R) RDT. The regional difference in the performance of rK39-based RDT and lower sensitivity in Leishmania/HIV coinfected patients raise concern on the routine use of these products for the diagnosis of VL.-
Descrição: dc.descriptionMinistry of Health, Brazil-
Descrição: dc.descriptionLaboratorio de Investigacao Medica Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniv Sao Paulo, Inst Med Trop, Lab Soroepidemiol & Imunobiol, Fac Med, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Med, Dept Doencas Infecciosas & Parasitarias, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionInst Infectol Emilio Ribas, Secretaria Estado Saude, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionUniv Fed Sergipe, Hosp Univ EBSERH, Dept Med Interna & Patol, Aracaju, SE, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Dept Doencas Trop & Diagnost Imagem, Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Fed Mato Grosso, Hosp Univ Maria Aparecida Pedrossian, Campo Grande, MS, Brazil-
Descrição: dc.descriptionUniv Fed Rio Grande do Norte, Hosp Univ Onofre Lopes, Natal, RN, Brazil-
Descrição: dc.descriptionHosp Giselda Trigueiro, Secretaria Estadual Seguranca Publ, Natal, RN, Brazil-
Descrição: dc.descriptionUniv Fed Mato Grosso do Sul, Fac Med, Campo Grande, MS, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Med, Dept Med Prevent, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Dept Doencas Trop & Diagnost Imagem, Botucatu, SP, Brazil-
Descrição: dc.descriptionMinistry of Health, Brazil: 084727/2010-
Descrição: dc.descriptionLaboratorio de Investigacao Medica Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo: LIM-38-
Formato: dc.format19-
Idioma: dc.languageen-
Publicador: dc.publisherPublic Library Science-
Relação: dc.relationPlos One-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titlePerformance of rK39-based immunochromatographic rapid diagnostic test for serodiagnosis of visceral leishmaniasis using whole blood, serum and oral fluid-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.