Boa.PLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorInst Butantan-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorBittencourt Rodrigues, Caroline Fabri-
Autor(es): dc.creatorSerino-Silva, Caroline-
Autor(es): dc.creatorMorais-Zani, Karen de-
Autor(es): dc.creatorKavazoi, Victor Koiti-
Autor(es): dc.creatorNogueira Carvalho, Marcelo Pires-
Autor(es): dc.creatorGrego, Kathleen Fernandes-
Autor(es): dc.creatorChiarelli, Tassia-
Autor(es): dc.creatorTashima, Alexandre Keiji-
Autor(es): dc.creatorToyama, Marcos Hikari [UNESP]-
Autor(es): dc.creatorTanaka-Azevedo, Anita Mitico-
Data de aceite: dc.date.accessioned2022-02-22T00:10:46Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:46Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-02-27-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0229657-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196909-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196909-
Descrição: dc.descriptionPlasma in several organisms has components that promote resistance to envenomation by inhibiting specific proteins from snake venoms, such as phospholipases A(2) (PLA(2)s). The major hypothesis for inhibitor's presence would be the protection against self-envenomation in venomous snakes, but the occurrence of inhibitors in non-venomous snakes and other animals has opened new perspectives for this molecule. Thus, this study showed for the first time the structural and functional characterization of the PLA(2) inhibitor from the Boa constrictor serum (Boa.PLI), a non-venomous snake that dwells extensively the Brazilian territory. Therefore, the inhibitor was isolated from B. constrictor serum, with 0.63% of recovery. SDS-PAGE showed a band at similar to 25 kDa under reducing conditions and similar to 20 kDa under non-reducing conditions. Chromatographic analyses showed the presence of oligomers formed by Boa.PLI. Primary structure of Boa.PLI suggested an estimated molecular mass of 22 kDa. When Boa.PLI was incubated with Asp-49 and Lys-49 PLA(2) there was no severe change in its dichroism spectrum, suggesting a non-covalent interaction. The enzymatic assay showed a dose-dependent inhibition, up to 48.2%, when Boa.PLI was incubated with Asp-49 PLA(2), since Lys-49 PLA(2) has a lack of enzymatic activity. The edematogenic and myotoxic effects of PLA(2)s were also inhibited by Boa.PLI. In summary, the present work provides new insights into inhibitors from non-venomous snakes, which possess PLIs in their plasma, although the contact with venom is unlikely.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Sao Paulo, Inst Pesquisas Tecnol, Inst Butantan, Interunidades Biotecnol, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionInst Butantan, Lab Herpetol, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionUniv Fed Minas Gerais, Belo Horizonte, MG, Brazil-
Descrição: dc.descriptionUniv Fed Sao Paulo, Dept Bioquim, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biociencias Litoral Paulista, Sao Vicente, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biociencias Litoral Paulista, Sao Vicente, SP, Brazil-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2017/16908-2-
Descrição: dc.descriptionFAPESP: 2017/20106-9-
Descrição: dc.descriptionFAPESP: 2018/25786-0-
Formato: dc.format13-
Idioma: dc.languageen-
Publicador: dc.publisherPublic Library Science-
Relação: dc.relationPlos One-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleBoa.PLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor-
Tipo de arquivo: dc.typelivro digital-
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