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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Univ Torino | - |
Autor(es): dc.contributor | Augusta Univ | - |
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.creator | Ferreira, Livia C. [UNESP] | - |
Autor(es): dc.creator | Orso, Francesca | - |
Autor(es): dc.creator | Dettori, Daniela | - |
Autor(es): dc.creator | Lacerda, Jessica Z. [UNESP] | - |
Autor(es): dc.creator | Borin, Thaiz F. | - |
Autor(es): dc.creator | Taverna, Daniela | - |
Autor(es): dc.creator | Zuccari, Debora A. P. C. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:10:44Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:10:44Z | - |
Data de envio: dc.date.issued | 2020-12-09 | - |
Data de envio: dc.date.issued | 2020-12-09 | - |
Data de envio: dc.date.issued | 2020-02-02 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1371/journal.pone.0228062 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/196896 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/196896 | - |
Descrição: dc.description | Melatonin, a hormone secreted by pineal gland, exerts antimetastatic effects by reducing tumor cell proliferation, migration and invasion. MicroRNAs (miRNAs) are small, non-coding RNAs that play a crucial role in regulation of gene expression and biological processes of the cells. Herein, we search for a link between the tumor/metastatic-suppressive actions of melatonin and miRNA expression in triple-negative breast cancer cells. We demonstrated that melatonin exerts its anti-tumor actions by reducing proliferation, migration and c-Myc expression of triple negative breast cancer cells. By using Taqman-based assays, we analyzed the expression levels of a set of miRNAs following melatonin treatment of triple negative breast cancer cells and we identified 17 differentially expressed miRNAs, 6 down-regulated and 11 up-regulated. We focused on the anti-metastatic miR-148b and the oncogenic miR-210 both up-regulated by melatonin treatment and studied the effect of their modulation on melatonin-mediated impairment of tumor progression. Surprisingly, when miR-148b or miR-210 were depleted in triple-negative breast cancer cells, using a specific miR-148b sponge or anti-miR-210, melatonin effects on migration inhibition and c-myc downregulation were still visible suggesting that the increase of miR-148b and miR-210 expression observed following melatonin treatment was not required for the efficacy of melatonin action. Nevertheless, ours results suggest that melatonin exhibit a compound for metastatic trait inhibition, especially in MDA-MB-231 breast cancer cells even if a direct link between modulation of expression of certain proteins or miRNAs and melatonin effects has still to be established. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Univ Estadual Paulista, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil | - |
Descrição: dc.description | Univ Torino, Mol Biotechnol Ctr MBC, Dept Mol Biotechnol & Hlth Sci, Turin, Italy | - |
Descrição: dc.description | Augusta Univ, Dept Biochem & Mol Biol, Tumor Angiogenesis Lab, Augusta, GA USA | - |
Descrição: dc.description | Fac Med Sao Jose do Rio Preto, Dept Mol Biol, Sao Jose Do Rio Preto, SP, Brazil | - |
Descrição: dc.description | Univ Estadual Paulista, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil | - |
Descrição: dc.description | FAPESP: 2015/04780-6 | - |
Formato: dc.format | 15 | - |
Idioma: dc.language | en | - |
Publicador: dc.publisher | Public Library Science | - |
Relação: dc.relation | Plos One | - |
???dc.source???: dc.source | Web of Science | - |
Título: dc.title | The role of melatonin on miRNAs modulation in triple-negative breast cancer cells | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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