Cortisol Directly Stimulates Spermatogonial Differentiation, Meiosis, and Spermiogenesis in Zebrafish (Danio rerio) Testicular Explants

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Calgary-
Autor(es): dc.creatorTovo-Neto, Aldo [UNESP]-
Autor(es): dc.creatorMartinez, Emanuel R. M. [UNESP]-
Autor(es): dc.creatorMelo, Aline G. [UNESP]-
Autor(es): dc.creatorDoretto, Lucas B. [UNESP]-
Autor(es): dc.creatorButzge, Arno J. [UNESP]-
Autor(es): dc.creatorRodrigues, Maira S. [UNESP]-
Autor(es): dc.creatorNakajima, Rafael T. [UNESP]-
Autor(es): dc.creatorHabibi, Hamid R.-
Autor(es): dc.creatorNobrega, Rafael H. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:10:37Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:37Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/biom10030429-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196847-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196847-
Descrição: dc.descriptionCortisol is the major endocrine factor mediating the inhibitory effects of stress on vertebrate reproduction. It is well known that cortisol affects reproduction by interacting with the hypothalamic-pituitary-gonads axis, leading to downstream inhibitory and stimulatory effects on gonads. However, the mechanisms are not fully understood. In this study, we provide novel data demonstrating the stimulatory effects of cortisol on spermatogenesis using an ex vivo organ culture system. The results revealed that cortisol treatment did not modulate basal androgen production, but it influenced transcript levels of a selected number of genes involved in the zebrafish testicular function ar (androgen receptor), star (steroidogenic acute regulatory), cyp17a1 (17 alpha-hydroxylase/17,20 lyase/17,20 desmolase), cyp11a2 (cytochrome P450, family 11, subfamily A, polypeptide 2), hsd11b2 (11-beta hydroxysteroid dehydrogenase), cyp2k22 (cytochrome P450, family 2, subfamily K, polypeptide 22), fkbp5 (FKBP prolyl isomerase 5), gr alpha (glucocorticoid receptor alpha), and gr beta (glucocorticoid receptor beta) in a short-term culture. We also showed that cortisol stimulates spermatogonial proliferation and differentiation in an androgen independent manner as well as promoting meiosis and spermiogenesis by increasing the number of spermatozoa in the testes. Moreover, we demonstrated that concomitant treatment with RU 486, a potent glucocorticoid receptor (Gr) antagonist, did not affect the cortisol effects on spermatogonial differentiation but blocked the induced effects on meiosis and spermiogenesis. Supporting the Gr-mediated effects, RU 486 nullified the cortisol-induced expression of sycp3l (synaptonemal complex protein 3), a marker for the meiotic prophase that encodes a component of the synaptonemal complex. This is consistent with in silico analysis that found 10 putative GREs (glucocorticoid response elements) upstream of the zebrafish sycp3l. Finally, we also showed that gr alpha mRNA is expressed in Sertoli and Leydig cells, but also in several types of germ cells, including spermatogonia and spermatocytes. Altogether, this evidence indicates that cortisol exerts paracrine roles in the zebrafish testicular function and spermatogenesis, highlighting its effects on spermatogonial differentiation, meiosis, and spermiogenesis.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada (NSERC)-
Descrição: dc.descriptionSao Paulo State Univ, Aquaculture Program CAUNESP, BR-14884900 Jaboticabal, SP, Brazil-
Descrição: dc.descriptionUniv Calgary, Dept Biol Sci, Calgary, AB T2N 1N4, Canada-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, Dept Morphol, Reprod & Mol Biol Grp, BR-18618970 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Calgary, Dept Physiol & Pharmacol, Calgary, AB T2N 1N4, Canada-
Descrição: dc.descriptionSao Paulo State Univ, Aquaculture Program CAUNESP, BR-14884900 Jaboticabal, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, Dept Morphol, Reprod & Mol Biol Grp, BR-18618970 Botucatu, SP, Brazil-
Descrição: dc.descriptionCNPq: 234548/2014-2-
Descrição: dc.descriptionFAPESP: 14/16280-5-
Descrição: dc.descriptionFAPESP: 17/09218-0-
Descrição: dc.descriptionFAPESP: 14/07620-7-
Descrição: dc.descriptionFAPESP: 15/12104-0-
Descrição: dc.descriptionFAPESP: 16/12101-4-
Descrição: dc.descriptionFAPESP: 17/15793-7-
Descrição: dc.descriptionFAPESP: 13/22778-3-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada (NSERC): 1021837-
Formato: dc.format24-
Idioma: dc.languageen-
Publicador: dc.publisherMdpi-
Relação: dc.relationBiomolecules-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectcortisol-
Palavras-chave: dc.subjectglucocorticoid receptor-
Palavras-chave: dc.subjectspermatogenesis-
Palavras-chave: dc.subjectzebrafish-
Título: dc.titleCortisol Directly Stimulates Spermatogonial Differentiation, Meiosis, and Spermiogenesis in Zebrafish (Danio rerio) Testicular Explants-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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