Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Fed Para-
Autor(es): dc.contributorUniversidade Estadual de Ponta Grossa (UEPG)-
Autor(es): dc.contributorUniv Fed Rural Amazonia-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorCavalcante, Manoella Gemaque-
Autor(es): dc.creatorSouza, Luciano Farias-
Autor(es): dc.creatorVicari, Marcelo Ricardo-
Autor(es): dc.creatorMatos de Bastos, Carlos Eduardo-
Autor(es): dc.creatorSousa, Jaime Viana de-
Autor(es): dc.creatorNagamachi, Cleusa Yoshiko-
Autor(es): dc.creatorPieczarka, Julio Cesar-
Autor(es): dc.creatorMartins, Cesar [UNESP]-
Autor(es): dc.creatorRodrigues Noronha, Renata Coelho-
Data de aceite: dc.date.accessioned2022-02-22T00:10:22Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:22Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-05-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.gene.2020.144477-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196753-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196753-
Descrição: dc.descriptionThe wide variation found in the size of eukaryotic genomes is largely related to the accumulation of repetitive sequences. Studies show that these sequences can go through an evolutionary process (molecular co-optation) and acquire new genomic functions. Cytogenetic studies reveal a wide karyotypic variation between chelonians (order Testudines) (2n = 26-68), attributed mainly to the number of microchromosomes. The study of repetitive DNAs has the potential to provide data on the dynamics of these sequences, and how they influence the organization of the genome. Here, we reveal the first in situ mapping data of 45S rDNA, histone H3 genes, and telomeric sequences, for a species of the genus Rhinoclemmys, R. punctularia. The karyotype described here for R. punctularia is different from previous reports for the diploid complement of this species, with differences probably attributable to centric fissions and pericentric inversions or centromere repositioning. The 45S rDNA are on a single chromosome pair (like in other turtles), telomeric sequences are in terminal position on all the chromosomes, and histone H3 is dispersed in low copy number, with clusters in pericentromeric regions of three chromosome pairs. We report on the presence of a Gypsy retrotransposon insert located within H3 histone of R. punctularia, and the H3 region sequenced contained the open reading frame of the histone sequence. Comparative modeling revealed a functional pattern for the protein, thus suggesting that the Gypsy element might have been recruited for new functions in the genome of this species.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionNational Bank for Economic and Social Development - BNDES-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Fed Para, Ctr Estudos Avancados Biodiversidade, Inst Ciencias Biol, Lab Citogenet, Belem, Para, Brazil-
Descrição: dc.descriptionUniv Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Ponta Grossa, Parana, Brazil-
Descrição: dc.descriptionUniv Fed Rural Amazonia, Campus Capanema, Capanema, Parana, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Dept Morfol, Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Dept Morfol, Botucatu, SP, Brazil-
Descrição: dc.descriptionCNPq: 475013/2012-3-
Descrição: dc.descriptionNational Bank for Economic and Social Development - BNDES: 2.318.697.0001-
Descrição: dc.descriptionCNPq: 47/2012-
Descrição: dc.descriptionCNPq: 305880/2017-9-
Descrição: dc.descriptionCNPq: 305876/2017-1-
Formato: dc.format7-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationGene-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectHistone genes-
Palavras-chave: dc.subjectRetrotransposon LTR-
Palavras-chave: dc.subject45S rDNA-
Palavras-chave: dc.subjectKaryotypic reorganization-
Palavras-chave: dc.subjectTurtle-
Título: dc.titleMolecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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