Molecular detection of Hepatozoon spp. in non-hematophagous bats in Brazil

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Catolica Dom Bosco-
Autor(es): dc.contributorUniversidade do Estado do Rio de Janeiro (UERJ)-
Autor(es): dc.creatorPerles, Livia [UNESP]-
Autor(es): dc.creatorIkeda, Priscila [UNESP]-
Autor(es): dc.creatorFrancisco, Gabriela de Vasconcellos [UNESP]-
Autor(es): dc.creatorTorres, Jaire Marinho [UNESP]-
Autor(es): dc.creatorOliveira, Carina Elisei de-
Autor(es): dc.creatorLourenco, Elizabete Captivo-
Autor(es): dc.creatorHerrera, Heitor Miraglia-
Autor(es): dc.creatorMachado, Rosangela Zacarias [UNESP]-
Autor(es): dc.creatorAndre, Marcos Rogerio [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:10:19Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:19Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2020.101401-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196739-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196739-
Descrição: dc.descriptionEven though Hepatozoon spp. has been molecularly detected in several wild animals in Brazil, there is no report on the occurrence of Hepatozoon spp. DNA in bats in Brazil. This study aimed at detecting Hepatozoon, in addition to ectoparasites, in non-hematophagous bats sampled in central-western Brazil using blood smears, hematoxylineosin (HE)-staining liver/spleen preparations and molecular and phylogenetic techniques. A total of 135 spleen, 127 liver, and 133 blood samples were collected from 135 non-hematophagous bats from 12 different species in two different sites in Campo Grande, Mato Grosso do Sul state, in the Brazilian Cerrado region. Spleen and blood DNA samples were submitted to two conventional PCR protocols for Hepatozoon spp. based on 18S rRNA. No Hepatozoon spp. gamonts or meronts were observed in blood smears and HE-stained-liver preparations, respectively. While none of the spleen samples was positive for Hepatozoon spp. in the PCR assays, 5 (3 %) blood samples contained 18S rRNA Hepatozoon DNA, including 2/37 (5 %) Artibeus lituratus, 2/32 (6 %) A. planirostris, and 1/23 (4 %) Platyrrhinus lineatus. Out of 5 bats positive for Hepatozoon spp., 3 were parasitized by either Macronyssidae/Spinturnicidae mites or Streblidae flies. BLAST analysis showed that the sequences detected in bats had > 99 % identity with Hepatozoon sequences detected in amphibians and reptiles from Brazil, including Hepatozoon caimani detected in Caiman crocodiles. The phylogenetic inferences estimated by the Maximum Likelihood and Bayesian methods clustered the Hepatozoon sequences detected in Brazilian bats with those detected in reptiles and amphibians.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniv Estadual Julio de Mesquita Filho, Dept Patol Vet, Lab Imunoparasitol, Jaboticabal, Brazil-
Descrição: dc.descriptionUniv Catolica Dom Bosco, Lab Ciencias Ambientais & Sustentabilidade Agr, Campo Grande, MS, Brazil-
Descrição: dc.descriptionUniv Estado Rio de Janeiro, Lab Ecol Mamiferos, Rio De Janeiro, Brazil-
Descrição: dc.descriptionUniv Estadual Julio de Mesquita Filho, Dept Patol Vet, Lab Imunoparasitol, Jaboticabal, Brazil-
Descrição: dc.descriptionFAPESP: 2018/02753-0-
Descrição: dc.descriptionFAPESP: 2019/15150-4-
Descrição: dc.descriptionFAPESP: 2019/13232-3-
Descrição: dc.descriptionFAPESP: 2017/14124-4-
Descrição: dc.descriptionFUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul): 59/300.187/2016-
Descrição: dc.descriptionCNPq: 302420/2017-7-
Formato: dc.format6-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationTicks And Tick-borne Diseases-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectChiroptera-
Palavras-chave: dc.subjectHepatozoonosis-
Palavras-chave: dc.subject18S rRNA-
Título: dc.titleMolecular detection of Hepatozoon spp. in non-hematophagous bats in Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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