Nanoimmunosensor based on atomic force spectroscopy to detect anti-myelin basic protein related to early-stage multiple sclerosis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorRheabiotech Lab Res & Dev-
Autor(es): dc.creatorGarcia, Pamela Soto-
Autor(es): dc.creatorBrum, Doralina Guimaraes [UNESP]-
Autor(es): dc.creatorOliveira Jr, Osvaldo N.-
Autor(es): dc.creatorHiga, Akemi Martins-
Autor(es): dc.creatorMagalhaes Ierich, Jessica Cristiane-
Autor(es): dc.creatorMoraes, Ariana de Souza-
Autor(es): dc.creatorShimizu, Flavio Makoto-
Autor(es): dc.creatorOkuda-Shinagawa, Nancy M.-
Autor(es): dc.creatorPeroni, Luis Antonio-
Autor(es): dc.creatorGama, Paulo Diniz da-
Autor(es): dc.creatorTeresa Machini, M.-
Autor(es): dc.creatorLeite, Fabio Lima-
Data de aceite: dc.date.accessioned2022-02-22T00:10:17Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:17Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ultramic.2020.112946-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196728-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196728-
Descrição: dc.descriptionMultiple Sclerosis (MS) is a chronic inflammatory disorder in the central nervous system for which biomarkers for diagnosis still remain unknown. One potential biomarker is the myelin basic protein. Here, a nanoimmunosensor based on atomic force spectroscopy (AFS) successfully detected autoantibodies against the MBP85-99 peptide from myelin basic protein. The nanoimmunosensor consisted of an atomic force microscope tip functionalization with MBP85-99 peptide, which was made to interact with a mica surface coated either with a layer of anti-MBP85-99 (positive control) or samples of cerebrospinal fluid (CSF) from five multiple sclerosis (MS) patients at different stages of the disease and five non-MS subjects. The adhesion forces obtained from AFS pointed to a high concentration of anti-MBP85-99 for the two patients at early stages of relapsing-remitting multiple sclerosis (RRMS), which were indistinguishable from the positive control. In contrast, considerably lower adhesion forces were measured for all the other eight subjects, including three MS patients with longer history of the disease and under treatment, without episodes of acute MS activity. We have also shown that the average adhesion force between MBP85-99 and anti-MBP85-99 is compatible with the value estimated using steered molecular dynamics. Though further tests will be required with a larger cohort of patients, the present results indicate that the nanoimmunosensor may be a simple tool to detect early-stage MS patients and be useful to understand the molecular mechanisms behind MS.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionNational Institute for Science and Technology on Organic Electronics(INEO)-
Descrição: dc.descriptionUniv Fed Sao Carlos, Dept Phys Chem & Math, Nanoneurobiophys Res Grp, BR-18052780 Sorocaba, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil-
Descrição: dc.descriptionRheabiotech Lab Res & Dev, BR-13084791 Campinas, SP, Brazil-
Descrição: dc.descriptionPontifical Catholic Univ Sao Paulo, BR-18030010 Sorocaba, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2013/14262-7-
Descrição: dc.descriptionFAPESP: 2014/21530-0-
Descrição: dc.descriptionFAPESP: 2015/14360-4-
Descrição: dc.descriptionFAPESP: 2015/06847-0-
Descrição: dc.descriptionFAPESP: 2015/36143-2-
Descrição: dc.descriptionFAPESP: 2015/052836-
Descrição: dc.descriptionFAPESP: 2014/12082-4-
Descrição: dc.descriptionFAPESP: 2012/50839-4-
Descrição: dc.descriptionFAPESP: 2014/50869-6-
Descrição: dc.descriptionCNPq: 465572/2014-6-
Descrição: dc.descriptionCNPq: 308658/2015-9-
Descrição: dc.descriptionCNPq: 459768/2014-0-
Descrição: dc.descriptionCAPES: 23038.000776/201754-
Formato: dc.format8-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationUltramicroscopy-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectNanoimmunosensor-
Palavras-chave: dc.subjectAtomic force spectroscopy-
Palavras-chave: dc.subjectSteered molecular dynamics-
Palavras-chave: dc.subjectMyelin basic protein-
Palavras-chave: dc.subjectMultiple sclerosis-
Palavras-chave: dc.subjectEarly-stage patients-
Título: dc.titleNanoimmunosensor based on atomic force spectroscopy to detect anti-myelin basic protein related to early-stage multiple sclerosis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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