Short communication: Investigation of extra-intestinal pathogenic Escherichia coli virulence genes, bacterial motility, and multidrug resistance pattern of strains isolated from dairy cows with different severity scores of clinicalmastitis

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorPaulista Agcy Agribusiness Technol-
Autor(es): dc.creatorGuerra, Simony T. [UNESP]-
Autor(es): dc.creatorOrsi, Henrique [UNESP]-
Autor(es): dc.creatorJoaquim, Samea F. [UNESP]-
Autor(es): dc.creatorGuimaraes, Felipe F. [UNESP]-
Autor(es): dc.creatorLopes, Bruna C. [UNESP]-
Autor(es): dc.creatorDalanezi, Felipe M. [UNESP]-
Autor(es): dc.creatorLeite, Domingos S.-
Autor(es): dc.creatorLangoni, Helio [UNESP]-
Autor(es): dc.creatorPantoja, Jose C. F. [UNESP]-
Autor(es): dc.creatorRall, Vera L. M. [UNESP]-
Autor(es): dc.creatorHernandes, Rodrigo T. [UNESP]-
Autor(es): dc.creatorLucheis, Simone B.-
Autor(es): dc.creatorRibeiro, Marcio G. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:10:11Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:11Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3168/jds.2019-17477-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196693-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196693-
Descrição: dc.descriptionEscherichia coli is a major pathogen involved in the etiology of environmentally derived bovine mastitis and is characterized by a variety of virulence factors (VF). Mammary infections with E. coli have shown a wide range of clinical signs, causing changes in milk (score 1, or mild), abnormal appearance of milk and udder inflammation (score 2, or moderate), and abnormalities in milk, udder inflammation, and systemic signs of illness (score 3, or severe). Nevertheless, to date, the profile of the genes related to the virulence of the pathogen in mammary infections arid the severity scores of cases have not been thoroughly elucidated. Therefore, a panel of 18 virulence-encoding genes associated with extra-enteric pathogenicity of E. coli (ExPEC) were investigated in addition to in vitro swimming and swarming motility profiles and antimicrobial susceptibility/resistance patterns among 114 E. coli strains isolated from cows with clinical mastitis and different severity scores. Of 114 clinical cases, 39.5, 54.4, and 6.1% were mild, moderate, and severe, respectively. The main genes related to VF harbored by isolates were adhesins (fimH 100%; ecpA 64.0%, firnA 31.6%), serum resistance (traT 81.6%; ompT 35.1%), siderophores (irp2 9.6%), and hemolysin (hlyA 7%). Among the isolates studied, 99.1% showed in vitro resistance to bacitracin and cloxacillin, and 98.2% to lincosamin. Of the total isolates, 98.2% were considered multidrug resistant based on the multiple antimicrobial resistance index. No significant difference was observed between mean swimming (13.8 mm) and swarming (13.5 mm) motility, as well as severity scores of clinical mastitis and the ExPEC genes studied. The isolation of strains resistant to various antimicrobials, even though tested only in vitro, highlights the importance of rational use of antimicrobials for mastitis treatment. The high prevalence of the genes related to serum resistance (traT and ompT) and adhesion (ecpA) of the pathogen, in addition to main associations between the genes fimH, ecpA, and traT among cows with severity scores of 1 (15%) arid 2 (22.6%), indicates that the genes traT, ecpA, and ompT could be further studied as biomarkers of ExPEC for clinical intramammary infections. In addition, the ExPEC genes ompT (protectin), ibe10 (invasiri), arid ecpA (adhesin) were investigated for the first time among cows with mastitis, where scores of clinical severity were assessed. Results of this study contribute to the characterization of virulence mechanisms and antimicrobial resistance profile of ExPEC variants that affect dairy cows with different scores of clinical mastitis.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Hyg & Publ Hlth, BR-18618681 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Microbiol & Immunol, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, Dept Genet Evolut Microbiol & Immunol, BR-13083970 Campinas, SP, Brazil-
Descrição: dc.descriptionPaulista Agcy Agribusiness Technol, BR-17030000 Bauru, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Hyg & Publ Hlth, BR-18618681 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Microbiol & Immunol, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2015/19688-8-
Descrição: dc.descriptionFAPESP: 2017/08102-8-
Formato: dc.format3606-3614-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationJournal Of Dairy Science-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectenvironmental mastitis-
Palavras-chave: dc.subjectextra-enteric pathogenicity of Escherichia coli-
Palavras-chave: dc.subjectmechanism of virulence-
Palavras-chave: dc.subjectmultidrug resistance-
Título: dc.titleShort communication: Investigation of extra-intestinal pathogenic Escherichia coli virulence genes, bacterial motility, and multidrug resistance pattern of strains isolated from dairy cows with different severity scores of clinicalmastitis-
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Aparece nas coleções:Repositório Institucional - Unesp

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