Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Austral Chile-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorQuercia, Camila A.-
Autor(es): dc.creatorSuarez-Villota, Elkin Y.-
Autor(es): dc.creatorForesti, Fausto [UNESP]-
Autor(es): dc.creatorNunez, Jose J.-
Data de aceite: dc.date.accessioned2022-02-22T00:10:05Z-
Data de disponibilização: dc.date.available2022-02-22T00:10:05Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-01-26-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3897/CompCytogen.v14i1.46852-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196653-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196653-
Descrição: dc.descriptionSouth American frogs of the genus Eupsophus Fitzinger, 1843 comprise 10 species. Two of them, Eupsophus vertebralis Grandison, 1961 and E. emiliopugini Formas, 1989 belong to the Eupsophus vertebralis group, exhibiting 2n = 28. Fundamental number differences between these species have been described using conventional chromosome staining of few specimens from only two localities. Here, classical techniques (Giemsa, C-handing, CMA(3)/DAPI handing, and Ag-NOR staining), and fluorescence in situ hybridization (FISH, with telomeric and 28S ribosomal probes), were applied on individuals of both species collected from 15 localities. We corroborate differences in fundamental numbers (FN) between E. vertebralis and E. emiliopugini through Giemsa staining and C-banding (FN = 54 and 56, respectively). No interstitial fluorescent signals, but clearly stained telomeric regions were detected by FISH using telomeric probe over spreads from both species. FISH with 28S rDNA probes and Ag-NOR staining confirmed the active nucleolus organizer regions signal on pair 5 for both species. Nevertheless, one E. emiliopugini individual from the Puyehue locality exhibited 28S ribosomal signals on pairs 4 and 5. Interestingly, only one chromosome of each pair showed Ag-NOR positive signals, showing a nucleolar dominance pattern. Chromosomal rearrangements, rRNA gene dosage control, mobile NORs elements, and/or species hybridization process could he involved in this interpopulation chromosomal variation.-
Descrição: dc.descriptionFondecyt-
Descrição: dc.descriptionCONICYT-
Descrição: dc.descriptionUniv Austral Chile, Inst Ciencias Marinas & Limnol, Edificio Emilio Pugin,Campus Isla Teja S-N, Valdivia, Chile-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP, Brazil-
Descrição: dc.descriptionFondecyt: 3160328-
Descrição: dc.descriptionCONICYT: 22180766-
Formato: dc.format61-74-
Idioma: dc.languageen-
Publicador: dc.publisherPensoft Publishers-
Relação: dc.relationComparative Cytogenetics-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectKaryotype variations-
Palavras-chave: dc.subjectFISH-
Palavras-chave: dc.subjectPatagonian frogs-
Palavras-chave: dc.subjectribosomal probe-
Palavras-chave: dc.subjectNOR polymorphism-
Título: dc.titleComparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.