The applicability of gene sequencing and MALDI-TOF to identify less common gram-negative rods (Advenella, Castellaniella, Kaistia, Pusillimonas and Sphingobacterium) from environmental isolates

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorBarretos Canc Hosp-
Autor(es): dc.contributorMicroBiol Sect-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorSao Francisco Univ-
Autor(es): dc.creatorSant' Anna, Debora-
Autor(es): dc.creatorSampaio, Jorge Luiz Mello-
Autor(es): dc.creatorSommaggio, Lais Roberta Deroldo [UNESP]-
Autor(es): dc.creatorMazzeo, Dania Elisa Christofoletti [UNESP]-
Autor(es): dc.creatorMarin-Morales, Maria Aparecida [UNESP]-
Autor(es): dc.creatorMarson, Fernando Augusto Lima-
Autor(es): dc.creatorLevy, Carlos Emilio-
Data de aceite: dc.date.accessioned2022-02-22T00:09:48Z-
Data de disponibilização: dc.date.available2022-02-22T00:09:48Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10482-019-01333-0-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196561-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196561-
Descrição: dc.descriptionOur aim was to identify less common non-fermenting gram-negative rods during the bioremediation process. Five genera were found: Advenella, Castellaniella, Kaistia, Pusillimonas and Sphingobacterium, for a total of 15 isolates. Therefore, we evaluated the applicability of four methods currently available for bacteria identification: (1) conventional biochemical methods, (2) the VITEK (R)-2 system, (3) MALDI-TOF mass spectrometry and (4) 16S rRNA gene sequencing. The biochemical methods and the VITEK (R)-2 system were reliable only for the Sphingobacterium isolate and solely at the genus level. Both MALDI-TOF mass spectrometry platforms (Bruker and VITEK (R) MS) did not achieve reliable identification results for any of these genera. 16S rRNA gene sequencing identified eight isolates to the species level but not to the subspecies level, when applicable. The remaining seven isolates were reliably identified through 16S rRNA gene sequencing to the genus level only. Our findings suggest that the detection and identification of less common genera (and species) that appeared at certain moments during the bioremediation process can be a challenge to microbiologists considering the most used techniques. In addition, more studies are required to confirm our results.-
Descrição: dc.descriptionUniv Estadual Campinas, Fac Med Sciences, Dept Clin Pathol, MicroBiol Lab, Rua Tessalia Vieira Camargo, 126, BR-13083-88 Campinas, SP, Brazil-
Descrição: dc.descriptionBarretos Canc Hosp, Mol Oncol Res Ctr, Rua Antenor Duarte Villela, 1331, Barretos, SP, Brazil-
Descrição: dc.descriptionMicroBiol Sect, Centers Diagnost Med, . Gen Waldomiro Lima 508, BR-04344-07 Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Sch Pharm, Toxicol Dept, Clin Anal, . Prof Lineu Prestes, 580, BR-05508-00 Butanta, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosciences, Dept Biol, . 24, 1515, Bela Vista, BR-13506-90 Rio Claro, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Chem, Dept Analyt Chem, Rua Prof Francisco Degni, 55, BR-14800-06 Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, Fac Med Sciences, Dept Pediat, Rua Tessalia Vieira Camargo, 126, BR-13083-88 Campinas, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, Fac Med Sciences, Ctr Pediat Invest, Lab Pulm Physiol, Rua Tessalia Vieira Camargo, 126, BR-13083-88 Campinas, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, Fac Med Sciences, Dept Med Genet, Gen Med, Rua Tessalia Vieira Camargo, 126, BR-13083-88 Campinas, SP, Brazil-
Descrição: dc.descriptionSao Francisco Univ, PostGrad Program Hlth Sci, Ave Sao Francisco Assis, 218, BR-12916-40 Braganca Paulista, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosciences, Dept Biol, . 24, 1515, Bela Vista, BR-13506-90 Rio Claro, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Chem, Dept Analyt Chem, Rua Prof Francisco Degni, 55, BR-14800-06 Araraquara, SP, Brazil-
Formato: dc.format233-252-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationAntonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subject16S rRNA-
Palavras-chave: dc.subjectAdvenella-
Palavras-chave: dc.subjectCastellaniella-
Palavras-chave: dc.subjectKaistia-
Palavras-chave: dc.subjectPusillimonas-
Palavras-chave: dc.subjectSphingobacterium-
Título: dc.titleThe applicability of gene sequencing and MALDI-TOF to identify less common gram-negative rods (Advenella, Castellaniella, Kaistia, Pusillimonas and Sphingobacterium) from environmental isolates-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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