Incorporating N-acetylcysteine and tricalcium phosphate into epoxy resin-based sealer improved its biocompatibility and adhesiveness to radicular dentine

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Florida-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Michigan-
Autor(es): dc.creatorRibeiro Camargo, Carlos Henrique [UNESP]-
Autor(es): dc.creatorLandim Gomes, Lais Carolina [UNESP]-
Autor(es): dc.creatorMoreira Franca, Monique Costa [UNESP]-
Autor(es): dc.creatorBittencourt, Tatiane Sampaio [UNESP]-
Autor(es): dc.creatorValera, Marcia Carneiro [UNESP]-
Autor(es): dc.creatorCamargo, Samira Esteves Afonso-
Autor(es): dc.creatorBottino, Marco Cicero-
Data de aceite: dc.date.accessioned2022-02-22T00:09:12Z-
Data de disponibilização: dc.date.available2022-02-22T00:09:12Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.dental.2019.09.001-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196355-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196355-
Descrição: dc.descriptionObjective. This in vitro study was designed to evaluate the biocompatibility, adhesiveness, and antimicrobial activity of epoxy resin-based sealer associated with N-Acetylcysteine (NAC) or beta-tricalcium phosphate nanoparticles (beta-TCP) as an experimental retro-filling material. Methods. Cytotoxicity was assessed using 2,3-Bis-(Methoxy-4-Nitro-5-Sulphophenyl)-2H- Tetrazolium-5-Carboxanilide (XTT) and Sulforhodamine B (SRB) assays after exposing human periodontal ligament fibroblasts to extracts of the materials for 1, 3, or 7 days. For the adhesive resistance test, root canals (48 single-root teeth) were instrumented with Reciproc #40 files (VDW GmbH, Germany) and obturated. After 7 days, the apices were sectioned and a retrograde cavity prepared and filled with the experimental materials (Mineral trioxide aggregate, Epoxy sealer, Epoxy sealer + NAC, and Epoxy sealer+ beta-TCP). For the push-out test, one 2-mm thick slice was obtained from the apical third of each specimen. Antimicrobial activity was performed using agar diffusion method. Biofilms were grown in microplates and exposed to the extracts of retro-filled materials, followed by analysis of growth inhibition on agar plates. Results. Epoxy sealer in association with beta-TCP or NAC showed better bond strength while Mineral trioxide aggregate allowed for the lowest adhesion. Mineral trioxide aggregate, Epoxy sealer+ beta-TCP, and Epoxy sealer + NAC showed low cytotoxicity. Epoxy sealer was the most cytotoxic. In antimicrobial activity assays, all materials had no effect on Candida albicans. Addition of NAC improved the antimicrobial property of Epoxy sealer against Enterococcus faecalis compared to unmodified Epoxy sealer (P < 0.05). Significance. Incorporating beta-TCP or NAC with Epoxy sealer could improve the adhesiveness and biocompatibility for better use in endodontic therapy. (C) 2019 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Florida, Coll Dent, Dept Restorat Dent Sci, Gainesville, FL USA-
Descrição: dc.descriptionSao Paulo State Univ, Sch Dent, Dept Restorat Dent, Sao Jose Dos Campos, SP, Brazil-
Descrição: dc.descriptionUniv Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA-
Descrição: dc.descriptionSao Paulo State Univ, Sch Dent, Dept Restorat Dent, Sao Jose Dos Campos, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2015/23324-1-
Formato: dc.format1750-1756-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationDental Materials-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectRadicular dentine-
Palavras-chave: dc.subjectAdhesion-
Palavras-chave: dc.subjectBiocompatibility-
Palavras-chave: dc.subjectEndodontics-
Palavras-chave: dc.subjectN-Acetylcysteine-
Palavras-chave: dc.subjectTricalcium phosphate-
Palavras-chave: dc.subjectRetro-Filling material-
Título: dc.titleIncorporating N-acetylcysteine and tricalcium phosphate into epoxy resin-based sealer improved its biocompatibility and adhesiveness to radicular dentine-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.