A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorRheabiotech Lab Res & Dev-
Autor(es): dc.creatorMoraes, Ariana de Souza-
Autor(es): dc.creatorBrum, Doralina Guimaraes [UNESP]-
Autor(es): dc.creatorMagalhaes Ierich, Jessica Cristiane-
Autor(es): dc.creatorHiga, Akemi Martins-
Autor(es): dc.creatorJabur Assis, Amanda Stefanie-
Autor(es): dc.creatorMiyazaki, Celina Massumi-
Autor(es): dc.creatorShimizu, Flavio Makoto-
Autor(es): dc.creatorPeroni, Luis Antonio-
Autor(es): dc.creatorMachini, M. Teresa-
Autor(es): dc.creatorBarreira, Amilton Antunes-
Autor(es): dc.creatorFerreira, Marystela-
Autor(es): dc.creatorOliveira Jr, Osvaldo N.-
Autor(es): dc.creatorLeite, Fabio Lima-
Data de aceite: dc.date.accessioned2022-02-22T00:09:02Z-
Data de disponibilização: dc.date.available2022-02-22T00:09:02Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-11-05-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-019-52506-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196300-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196300-
Descrição: dc.descriptionA precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients' prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4(61-70), AQP4(131-140), AQP4(141-150), and AQP4(201-210)) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP4(61-70)-nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP4(61-70) sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP4(61-70)-nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionBrazil National Council for Scientific and Technological Development-
Descrição: dc.descriptionNational Institute for Science and Technology on Organic Electronics -INEO-
Descrição: dc.descriptionUniv Sao Paulo, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo, SP, Brazil-
Descrição: dc.descriptionUniv Fed Sao Carlos, Dept Phys Chem & Math, BR-18052780 Sorocaba, SP, Brazil-
Descrição: dc.descriptionUniv Fed Sao Carlos, Nanoneurobiophys Res Grp GNN, BR-18052780 Sorocaba, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP, Brazil-
Descrição: dc.descriptionRheabiotech Lab Res & Dev, Campinas, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav Sci, Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: FAPESP 2013/14262-7-
Descrição: dc.descriptionFAPESP: 2015/05283-6-
Descrição: dc.descriptionFAPESP: 2015/06847-0-
Descrição: dc.descriptionFAPESP: 2014/12082-4-
Descrição: dc.descriptionFAPESP: 2014/15093-7-
Descrição: dc.descriptionFAPESP: 2016/19387-0-
Descrição: dc.descriptionFAPESP: 2015/36143-2-
Descrição: dc.descriptionFAPESP: 2015/14360-4-
Descrição: dc.descriptionFAPESP: 2012/50839-4-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionBrazil National Council for Scientific and Technological Development: CNPq 305069/2016-0-
Descrição: dc.descriptionBrazil National Council for Scientific and Technological Development: 459768/2014-0-
Descrição: dc.descriptionBrazil National Council for Scientific and Technological Development: 308570/2018-9-
Descrição: dc.descriptionBrazil National Council for Scientific and Technological Development: 308658/2015-9-
Descrição: dc.descriptionNational Institute for Science and Technology on Organic Electronics -INEO: CNPq 465572/2014-6-
Descrição: dc.descriptionNational Institute for Science and Technology on Organic Electronics -INEO: FAPESP 2014/50869-6-
Descrição: dc.descriptionNational Institute for Science and Technology on Organic Electronics -INEO: CAPES 23038.000776/201754-
Formato: dc.format9-
Idioma: dc.languageen-
Publicador: dc.publisherNature Publishing Group-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleA highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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