Biophysical characterization and molecular phylogeny of human KIN protein

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Maringá (UEM)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Fed Parana-
Autor(es): dc.creatorPattaro Junior, Jose Renato-
Autor(es): dc.creatorCaruso, Icaro Putinhon [UNESP]-
Autor(es): dc.creatorLima Neto, Quirino Alves de-
Autor(es): dc.creatorDuarte Junior, Francisco Ferreira-
Autor(es): dc.creatorRando, Fabiana dos Santos-
Autor(es): dc.creatorMarques Gerhardt, Edileusa Cristina-
Autor(es): dc.creatorFernandez, Maria Aparecida-
Autor(es): dc.creatorVicente Seixas, Flavio Augusto-
Data de aceite: dc.date.accessioned2022-02-22T00:08:43Z-
Data de disponibilização: dc.date.available2022-02-22T00:08:43Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00249-019-01390-3-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196185-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196185-
Descrição: dc.descriptionThe DNA/RNA-binding KIN protein was discovered in 1989, and since then, it has been found to participate in several processes, e.g., as a transcription factor in bacteria, yeasts, and plants, in immunoglobulin isotype switching, and in the repair and resolution of double-strand breaks caused by ionizing radiation. However, the complete three-dimensional structure and biophysical properties of KIN remain important information for clarifying its function and to help elucidate mechanisms associated with it not yet completely understood. The present study provides data on phylogenetic analyses of the different domains, as well as a biophysical characterization of the human KIN protein (HSAKIN) using bioinformatics techniques, circular dichroism spectroscopy, and differential scanning calorimetry to estimate the composition of secondary structure elements; further studies were performed to determine the biophysical parameters Delta H-m and T-m. The phylogenetic analysis indicated that the zinc-finger and winged helix domains are highly conserved in KIN, with mean identity of 90.37% and 65.36%, respectively. The KOW motif was conserved only among the higher eukaryotes, indicating that this motif emerged later on the evolutionary timescale. HSAKIN has more than 50% of its secondary structure composed by random coil and beta-turns. The highest values of Delta H-m and T-m were found at pH 7.4 suggesting a stable structure at physiological conditions. The characteristics found for HSAKIN are primarily due to its relatively low composition of alpha-helices and beta-strands, making up less than half of the protein structure.-
Descrição: dc.descriptionFundacao Araucaria-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniv Estadual Maringa, Dept Technol, Av Angelo Moreira da Fonseca 1800, BR-87506370 Umuarama, PR, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Phys, Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Maringa, Dept Biotechnol Genet & Cell Biol, Maringa, Parana, Brazil-
Descrição: dc.descriptionUniv Estadual Maringa, Ctr Mol Struct & Funct Biol, CBM Res Support Ctr Complex, Maringa, Parana, Brazil-
Descrição: dc.descriptionUniv Fed Parana, Dept Biochem, Curitiba, Parana, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Phys, Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionFundacao Araucaria: 147/14-
Descrição: dc.descriptionFundacao Araucaria: 40/16-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCNPq: 305960/2015-6-
Formato: dc.format645-657-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationEuropean Biophysics Journal With Biophysics Letters-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectKIN (Kin17) protein-
Palavras-chave: dc.subjectPhylogeny-
Palavras-chave: dc.subjectCircular dichroism-
Palavras-chave: dc.subjectDSC analysis-
Palavras-chave: dc.subjectTumor marker-
Título: dc.titleBiophysical characterization and molecular phylogeny of human KIN protein-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.