The Impact of Immunosuppressive Drugs on Human Placental Explants

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorGomes, Sara Z.-
Autor(es): dc.creatorAraujo, Franciele-
Autor(es): dc.creatorBandeira, Carla L.-
Autor(es): dc.creatorOliveira, Leandro G. [UNESP]-
Autor(es): dc.creatorHoshida, Mara S.-
Autor(es): dc.creatorZugaib, Marcelo-
Autor(es): dc.creatorFrancisco, Rossana P. V.-
Autor(es): dc.creatorBevilacqua, Estela-
Data de aceite: dc.date.accessioned2022-02-22T00:08:42Z-
Data de disponibilização: dc.date.available2022-02-22T00:08:42Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1177/1933719118812739-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196182-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196182-
Descrição: dc.descriptionThe use of immunosuppressive drugs guarantees the vitality of the graft and allows gestation in spite of intercurrences such as prematurity and intrauterine growth restriction. However, little is known about the direct effects of immunosuppressive drugs on placental cells. We investigated the effects of immunosuppressive drugs in the chorionic villous explants from human term placentas of healthy gestations. Human placental explants from term gestations (37-39 week gestational age, n = 12) were exposed to cyclosporine A (CSA, 0, 62.5, 125, 1250 ng/mL) or azathioprine (AZA, 0, 5, 10, 100 ng/mL) separately or, in combination for up to 48 hours. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed a significant decrease in the explant metabolic activity between AZA and the control group (24 hours, 100 ng/mL, 48 hours, all concentrations, P < .005). Cyclosporin A (CsA) reduced cell activity when associated with AZA (48 hours, P < .005). Fibrinoid deposits increased in AZA-treated explants alone (5 ng/mL, 48 hours; 10 ng/mL, 24-48 hours; P < .005) or when associated with CsA (10 AZA/125 CsA, P < .05), whereas in CsA treatment alone, there was an augment in syncytial knots (24-48 hours, P < .005). The sFLT1 gene (24 hours, P < .05) and protein (P < .005) expression increased in AZA and CsA-treatments separately or in combination (P < .05). Placental growth factor increased in AZA (24 hours, 10 ng/mL) and CsA (125 ng/mL; P < .05). In conclusion, our data indicate that AZA primarily acts on the villous metabolism, perturbing placental homeostasis. Since these drugs may alter the balance of angiogenic factors in its selection for clinical application, their impact on the behavior of placental villous should be considered.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniv Sao Paulo, Inst Biomed Sci, Dept Cellular & Dev Biol, Av Prof LineuPrestes 1524, BR-05508 Sao Paulo, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Botucatu Med Sch, Gynecol & Obstet Dept, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Obstet & Gynecol Dept, Med Sch, Sao Paulo, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Botucatu Med Sch, Gynecol & Obstet Dept, Sao Paulo, Brazil-
Formato: dc.format1225-1234-
Idioma: dc.languageen-
Publicador: dc.publisherSage Publications Inc-
Relação: dc.relationReproductive Sciences-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectplacenta-
Palavras-chave: dc.subjectexplants-
Palavras-chave: dc.subjectsFLT1-
Palavras-chave: dc.subjectPlGF-
Palavras-chave: dc.subjectcyclosporine-
Palavras-chave: dc.subjectazathioprine-
Título: dc.titleThe Impact of Immunosuppressive Drugs on Human Placental Explants-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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