The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorInst Oswaldo Cruz FIOCRUZ-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMimura, Kallyne K.-
Autor(es): dc.creatorTedesco, Roberto C.-
Autor(es): dc.creatorCalabrese, Katia S.-
Autor(es): dc.creatorGil, Cristiane D.-
Autor(es): dc.creatorOliani, Sonia M. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:08:07Z-
Data de disponibilização: dc.date.available2022-02-22T00:08:07Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2012-06-15-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/196004-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/196004-
Descrição: dc.descriptionPurpose: The aim of this study was to evaluate the expression of the protein annexin A1 (ANXA1), a potent endogenous regulator of the inflammatory process, in ocular toxoplasmosis. Methods: C57BL/ 6 female mice were infected using intravitreal injections of either 10(6) tachyzoites of Toxoplasma gondii (RH strain; T. gondii) or PBS only (control groups). After 24, 48, and 72 h, animals were sacrificed and their eyes were harvested for histopathological, immunohistochemical, and ultrastructural immunocytochemical analysis of ANXA1. Human retinal pigment epithelial (RPE) cells (ARPE-19) were infected in vitro with T. gondii and collected after 60, 120, 240 min, and 24 h. Results: Compared with non-infected eyes, an intense inflammatory response was observed in the anterior (24 h after infection) and posterior segments (72 h after infection) of the infected eye, characterized by neutrophil infiltration and by the presence of tachyzoites and their consequent destruction along with disorganization of normal retina architecture and RPE vacuolization. T. gondii infection was associated with a significant increase of ANXA1 expression in the neutrophils at 24, 48, and 72 h, and in the RPE at 48 and 72 h. In vitro studies confirmed an upregulation of ANXA1 levels in RPE cells, after 60 and 120 min of infection with T. gondii. Conclusions: The positive modulation of endogenous ANXA1 in the inflammatory and RPE cells during T. gondii infection suggests that this protein may serve as a therapeutic target in ocular toxoplasmosis.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, Sao Paulo, Brazil-
Descrição: dc.descriptionInst Oswaldo Cruz FIOCRUZ, Lab Imunomodulacao & Protozool, Rio De Janeiro, RJ, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Inst Biociencias Letras & Ciencias Exatas IBILCE, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Inst Biociencias Letras & Ciencias Exatas IBILCE, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2011/00128-1-
Descrição: dc.descriptionFAPESP: 2009/15240-1-
Descrição: dc.descriptionCNPq: 302768/2010-6-
Formato: dc.format1583-1593-
Idioma: dc.languageen-
Publicador: dc.publisherMolecular Vision-
Relação: dc.relationMolecular Vision-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleThe involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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