Optimization of cyclodextrin glucanotransferase production from Bacillus clausii E16 in submerged fermentation using response surface methodology

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAlves-Prado, Heloiza Ferreira-
Autor(es): dc.creatorBocchini, Daniela Alonso-
Autor(es): dc.creatorGomes, Eleni-
Autor(es): dc.creatorBaida, Luis Carlos-
Autor(es): dc.creatorContiero, Jonas-
Autor(es): dc.creatorRoberto, Ines Conceicao-
Autor(es): dc.creatorDa Silva, Roberto-
Data de aceite: dc.date.accessioned2021-03-10T17:14:25Z-
Data de disponibilização: dc.date.available2021-03-10T17:14:25Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2007-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s12010-007-9037-3-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/19565-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/19565-
Descrição: dc.descriptionCyclodextrin glucanotransferase production from Bacillus clausii E16, a new bacteria isolated from Brazilian soil samples was optimized in shake-flask cultures. A 2 4 full-factorial central composite design was performed to optimize the culture conditions, using a response surface methodology the combined effect among the soluble starch concentration, the peptone concentration, the yeast extract concentration, and the initial pH value of the culture medium was investigated. The optimum concentrations of the components, determined by a 2(4) full-factorial central composite design, were 13.4 g/L soluble starch, 4.9 g/L peptone, 5.9 g/L yeast extract, and initial pH 10.1. Under these optimized conditions, the maximum cyclodextrin glucanotransferase activity was 5.9 U/mL after a 48-h fermentation. This yield was 68% higher than that obtained when the microorganism was cultivated in basal culture medium.-
Formato: dc.format27-40-
Idioma: dc.languageen-
Publicador: dc.publisherHumana Press Inc-
Relação: dc.relationApplied Biochemistry and Biotechnology-
Relação: dc.relation1.797-
Relação: dc.relation0,571-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectalkalophillus Bacillus clausii-
Palavras-chave: dc.subjectCGTase production-
Palavras-chave: dc.subjectcyclodextrin glucanotransferase-
Palavras-chave: dc.subjectexperimental design-
Palavras-chave: dc.subjectsubmerged fermentation-
Palavras-chave: dc.subjectresponse surface methodology-
Título: dc.titleOptimization of cyclodextrin glucanotransferase production from Bacillus clausii E16 in submerged fermentation using response surface methodology-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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