Cholinergic regulation along the pulmonary arterial tree of the South American rattlesnake: vascular reactivity, muscarinic receptors, and vagal innervation

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Autor(es): dc.contributorAarhus Univ-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Estadual Ceara-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniv Birmingham-
Autor(es): dc.creatorFilogonio, Renato-
Autor(es): dc.creatorSartori, Marina R. [UNESP]-
Autor(es): dc.creatorMorgensen, Susie-
Autor(es): dc.creatorTavares, Driele-
Autor(es): dc.creatorCampos, Rafael-
Autor(es): dc.creatorAbe, Augusto S. [UNESP]-
Autor(es): dc.creatorTaylor, Edwin W.-
Autor(es): dc.creatorRodrigues, Gerson J.-
Autor(es): dc.creatorDe Nucci, Gilberto-
Autor(es): dc.creatorSimonsen, Ulf-
Autor(es): dc.creatorLeite, Cleo A. C.-
Autor(es): dc.creatorWang, Tobias-
Data de aceite: dc.date.accessioned2022-02-22T00:06:18Z-
Data de disponibilização: dc.date.available2022-02-22T00:06:18Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1152/ajpregu.00310.2019-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195606-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195606-
Descrição: dc.descriptionVascular tone in the reptil-ian pulmonary vasculature is primarily under cholinergic, muscarinic control exerted via the vagus nerve. This control has been ascribed to a sphincter located at the arterial outflow, but we speculated whether the vascular control in the pulmonary artery is more widespread, such that responses to acetylcholine and electrical stimulation, as well as the expression of muscarinic receptors, are prevalent along its length. Working on the South American rattlesnake (Crotalus durissus), we studied four different portions of the pulmonary artery (truncus, proximal, distal, and branches). Acetylcholine elicited robust vaso-constriction in the proximal, distal, and branch portions, but the truncus vasodilated. Electrical field stimulation (EFS) caused contrac-tions in all segments, an effect partially blocked by atropine. We identified all five subtypes of muscarinic receptors (M1-M5). The expression of the M1 receptor was largest in the distal end and branches of the pulmonary artery, whereas expression of the musca-rinic M3 receptor was markedly larger in the truncus of the pulmonary artery. Application of the neural tracer 1,1'-dioctadecyl-3,3,3',3'- tetramethylindo-carbocyanine perchlorate (DiI) revealed widespread innervation along the whole pulmonary artery, and retrograde trans-port of the same tracer indicated two separate locations in the brainstem providing vagal innervation of the pulmonary artery, the medial dorsal motor nucleus of the vagus and a ventro-lateral location, possibly constituting a nucleus ambiguus. These results revealed parasympathetic innervation of a large portion of the pulmonary artery, which is responsible for regulation of vascular conductance in C. durissus, and implied its integration with cardiorespiratory control.-
Descrição: dc.descriptionInstituto Nacional de Ciencia e Tecnologia em Fisiologia Comparada (INCT-FisC)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDanish Research Council-
Descrição: dc.descriptionScience without Borders program-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionAarhus Univ, Dept Biosci, Zoophysiol, Aarhus, Denmark-
Descrição: dc.descriptionUniv Fed Sao Carlos, Dept Physiol Sci, Sao Carlos, SP, Brazil-
Descrição: dc.descriptionState Univ Sao Paulo, Dept Zool, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionAarhus Univ, Dept Biomed Pulm & Cardiovasc Pharmacol, Aarhus, Denmark-
Descrição: dc.descriptionUniv Estadual Ceara, Super Inst Biomed Sci, Fortaleza, Ceara, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, Dept Pharmacol, Fac Med Sci, Campinas, SP, Brazil-
Descrição: dc.descriptionUniv Birmingham, Sch Biosci, Birmingham, W Midlands, England-
Descrição: dc.descriptionState Univ Sao Paulo, Dept Zool, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionScience without Borders program: 8938-13-0-
Descrição: dc.descriptionScience without Borders program: 401061/2014-0-
Descrição: dc.descriptionFAPESP: 2012/16537-0-
Descrição: dc.descriptionCNPq: 168061/2017-1-
Formato: dc.formatR156-R170-
Idioma: dc.languageen-
Publicador: dc.publisherAmer Physiological Soc-
Relação: dc.relationAmerican Journal Of Physiology-regulatory Integrative And Comparative Physiology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectcardiorespiratory integration-
Palavras-chave: dc.subjectelectrical field stimulation-
Palavras-chave: dc.subjectintracardiac shunts-
Palavras-chave: dc.subjectrespiratory sinus arrythmia-
Palavras-chave: dc.subjectvagus nerve-
Título: dc.titleCholinergic regulation along the pulmonary arterial tree of the South American rattlesnake: vascular reactivity, muscarinic receptors, and vagal innervation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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