Is the ADP ribose site of the Chikungunya virus NSP3 Macro domain a target for antiviral approaches?

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniv Leeds-
Autor(es): dc.contributorUniv Tartu-
Autor(es): dc.creatorShimizu, Jacqueline Farinha [UNESP]-
Autor(es): dc.creatorSilva Martins, Daniel Oliveira [UNESP]-
Autor(es): dc.creatorMcPhillie, Martin J.-
Autor(es): dc.creatorRoberts, Grace C.-
Autor(es): dc.creatorZothner, Carsten-
Autor(es): dc.creatorMerits, Andres-
Autor(es): dc.creatorHarris, Mark-
Autor(es): dc.creatorGomes Jardim, Ana Carolina [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:05:54Z-
Data de disponibilização: dc.date.available2022-02-22T00:05:54Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2020.105490-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195466-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195466-
Descrição: dc.descriptionChikungunya virus (CHIKV) is a mosquito-transmitted virus of special concern as it causes Chikungunya fever, characterized by an acute febrile illness, rash, and arthralgia that can progress to chronic and debilitating arthritic symptoms. The effects of climate change on the geographic distribution of the mosquito vector has the potential to expose more of the globe to this virus. No antiviral agents or vaccines are currently available against CHIKV infection and the development of novel therapies that may lead to a future treatment is therefore necessary. In this context, the ADP-ribose binding site of the CHIKV nsP3 macro domain has been reported as a potential target for the development of antivirals. Mutations in the ADP-ribose binding site demonstrated decreased viral replication in cell culture and reduced virulence. In this study, 48,750 small molecules were screened in silico for their ability to bind to the ADP-ribose binding site of the CHIKV nsP3 macro domain. From this in silico analysis, 12 molecules were selected for in vitro analysis using a CHIKV subgenomic replicon in Huh-7 cells. Cell viability and CHIKV replication were evaluated and molecules C5 and C13 demonstrated 53 and 66% inhibition of CHIKV replication, respectively. By using a CHIKV-Dual luciferase replicon contain two reporter genes, we also demonstrated that the treatment with either compounds are probably interfering in the early replication rather than after RNA replication has occurred.-
Descrição: dc.descriptionRoyal Society - Newton Advanced Fellowship-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionWellcome Trust-
Descrição: dc.descriptionSao Paulo State Univ, IBILCE, Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionUniv Fed Uberlandia, Inst Biomed Sci, Lab Virol, ICBIM, Uberlandia, MG, Brazil-
Descrição: dc.descriptionUniv Leeds, Fac Engn & Phys Sci, Sch Chem, Leeds LS2 9JT, W Yorkshire, England-
Descrição: dc.descriptionUniv Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England-
Descrição: dc.descriptionUniv Leeds, Fac Biol Sci, Sch Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England-
Descrição: dc.descriptionUniv Tartu, Inst Technol, Nooruse 1, EE-50411 Tartu, Estonia-
Descrição: dc.descriptionSao Paulo State Univ, IBILCE, Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionRoyal Society - Newton Advanced Fellowship: NA 150195-
Descrição: dc.descriptionCNPq: 445021/2014-4-
Descrição: dc.descriptionFAPEMIG: APQ-00587-14-
Descrição: dc.descriptionFAPEMIG: SICONV 793988/2013-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionWellcome Trust: 096670-
Formato: dc.format7-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationActa Tropica-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectChikungunya virus-
Palavras-chave: dc.subjectAntiviral-
Palavras-chave: dc.subjectnsP3-
Palavras-chave: dc.subjectMacro domain-
Título: dc.titleIs the ADP ribose site of the Chikungunya virus NSP3 Macro domain a target for antiviral approaches?-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.