Initial steps in selenocysteine biosynthesis: The interaction between selenocysteine lyase and selenophosphate synthetase

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniv British Columbia-
Autor(es): dc.contributorUniv Toronto-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorScortecci, Jessica F.-
Autor(es): dc.creatorSerrao, Vitor Hugo B.-
Autor(es): dc.creatorFernandes, Adriano F.-
Autor(es): dc.creatorBasso, Luis G. M.-
Autor(es): dc.creatorGutierrez, Raissa F.-
Autor(es): dc.creatorAraujo, Ana Paula U.-
Autor(es): dc.creatorNeto, Mario O. [UNESP]-
Autor(es): dc.creatorThiemann, Otavio H.-
Data de aceite: dc.date.accessioned2022-02-22T00:05:46Z-
Data de disponibilização: dc.date.available2022-02-22T00:05:46Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2020.03.241-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195422-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195422-
Descrição: dc.descriptionThe selenocysteine (Sec) incorporation is a co-translational event taking place at an in-frame UGA-codon and dependent on an organized molecular machinery. Selenium delivery requires mainly two enzymes, the selenocysteine lyase (CsdB) is essential for Sec recycling and conversion to selenide, further used by the selenophosphate synthetase (SelD), responsible for the conversion of selenide in selenophosphate. Therefore, understanding the catalytic mechanism involved in selenium compounds delivery, such as the interaction between SelD and CsdB (EcCsdB.EcSelD), is fundamental for the further comprehension of the selenocysteine synthesis pathway and its control. In Escherichia coli, EcCsdB.EcSelD interaction must occur to prevent cell death from the release of the toxic intermediate selenide. Here, we demonstrate and characterize the in vitro EcSelD.EcCsdB interaction by biophysical methods.The EcSelD.EcCsdB interaction occurs with a stoichiometiy of 1:1 in presence of selenocysteine and at a low-nanomolar affinity (similar to 1.8 nM). The data is in agreement with the small angle X-ray scattering model fitted using available structures. Moreover, yeast-2-hybrid assays supported the macromolecular interaction in the cellular environment. This is the first report that demonstrates the interaction between EcCsdB and EcSelD supporting the hypothesis that EcSelD.EcCsdB interaction is necessary to sequester the selenide during the selenocysteine incorporation pathway in Bacteria. (C) 2020 Elsevier B.V. All rights reserved.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniv Sao Paulo, Phys Inst Sao Carlos, Trabalhador Sao Carlense Av 400, BR-13566590 Sao Carlos, SP, Brazil-
Descrição: dc.descriptionUniv British Columbia, Dept Biochem & Mol Biol, 2350 Hlth Sci Mall, Vancouver, BC V6T 1Z3, Canada-
Descrição: dc.descriptionUniv Toronto, Lab Med & Pathobiol, 1 Kings Coll Circle, Toronto, ON M5S 1A8, Canada-
Descrição: dc.descriptionUniv Sao Paulo, Fac Sci Philosophy & Letters, BR-14040901 Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Biosci Inst, BR-18618000 Botucatu, SP, Brazil-
Descrição: dc.descriptionFed Univ Sao Carlos UFSCar, Dept Genet & Evolut, BR-13565905 Sao Carlos, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Biosci Inst, BR-18618000 Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2013/17791-0-
Descrição: dc.descriptionFAPESP: 2014/16005-4-
Descrição: dc.descriptionFAPESP: 2016/20977-7-
Descrição: dc.descriptionFAPESP: JFS 2012/23730-1-
Descrição: dc.descriptionFAPESP: 2014/00206-0-
Descrição: dc.descriptionCNPq: 420226/2016-8-
Formato: dc.format18-26-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationInternational Journal Of Biological Macromolecules-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectSelenophosphate synthetase-
Palavras-chave: dc.subjectSelenocysteine lyase-
Palavras-chave: dc.subjectSelenocysteine biosynthesis-
Título: dc.titleInitial steps in selenocysteine biosynthesis: The interaction between selenocysteine lyase and selenophosphate synthetase-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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