Photo-Induced Necrosis on Oropharyngeal Carcinoma (HEp-2) Cells Mediated by the Xanthene Erythrosine

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorBistaffa, Maria J. [UNESP]-
Autor(es): dc.creatorKobal, Mirella B. [UNESP]-
Autor(es): dc.creatorSouza, Priscila S. S. [UNESP]-
Autor(es): dc.creatorToledo, Karina A. [UNESP]-
Autor(es): dc.creatorCamacho, Sabrina A. [UNESP]-
Autor(es): dc.creatorAoki, Pedro H. B. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:05:38Z-
Data de disponibilização: dc.date.available2022-02-22T00:05:38Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1166/jnn.2020.18123-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195368-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195368-
Descrição: dc.descriptionThe photodynamic therapy (PDT) has been outstanding as a promising alternative for treating different carcinomas. However, the lack of detailed knowledge on the mechanisms of action prevents exploitation of the therapy full potential. Herein we shall evaluate not only the photodynamic efficiency but the mechanism of cell death triggered by the photoactivated erythrosine in oropharyngeal cancer cells (HEp-2). Cytotoxic assays were performed by MTT at distinct concentrations (10(-3) to 10(-6) mol/L) and incubation time (3, 24 and 48 h) of erythrosine in HEp-2 in vitro culture. In addition to the cytotoxic effect, the mechanisms of cell death were evaluated by flow cytometry following the annexin V/propidium iodide double staining protocol. Erythrosine was incorporated by HEp-2 cells in a dose- and time-dependent pathway. The incubation of erythrosine in dark has not shown any significant effect over the culture until 24 h and 1.25 x 10(-6) mol/L concentration, from which a small portion (<25% and statistically significant) of the cell population have undergone apoptosis. On the other hand, 50% of cell viability is reduced mainly by necrosis when 10, 3.75 and 1.9 x 10(-6) mol/L of erythrosine concentrations at 3, 24 and 48 h of incubation are photoactivated, respectively. Bioinspired models of tumor membrane based on Langmuir monolayers of 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) mixture reveled that electrostatic interactions with the lipid head groups are the main driving forces allowing the erythrosine adsorption. Furthermore, light-induced hydroperoxidation significantly increased the surface area of the monolayers, which might be the origin of the necrotic pathway triggered in HEp-2 cells.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSao Paulo State Univ, Sch Sci Humanities & Languages, UNESP, BR-19806900 Assis, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci Letters & Exact Sci, UNESP, BR-15054000 Sao Jose Do Rio Preto, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Sci Humanities & Languages, UNESP, BR-19806900 Assis, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci Letters & Exact Sci, UNESP, BR-15054000 Sao Jose Do Rio Preto, Brazil-
Descrição: dc.descriptionFAPESP: 2018/16713-0-
Descrição: dc.descriptionFAPESP: 2016/20576-2-
Descrição: dc.descriptionFAPESP: 2018/14692-5-
Descrição: dc.descriptionFAPESP: EMU 2013/14262-7-
Descrição: dc.descriptionFAPESP: EMU 04/09448-5-
Descrição: dc.descriptionCNPq: Universal 403713/2016-1-
Formato: dc.format6180-6190-
Idioma: dc.languageen-
Publicador: dc.publisherAmer Scientific Publishers-
Relação: dc.relationJournal Of Nanoscience And Nanotechnology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectPhotodynamic Therapy-
Palavras-chave: dc.subjectXanthene-
Palavras-chave: dc.subjectErythrosine B-
Palavras-chave: dc.subjectTumor Cells-
Título: dc.titlePhoto-Induced Necrosis on Oropharyngeal Carcinoma (HEp-2) Cells Mediated by the Xanthene Erythrosine-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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