Recombinant xylanase production by Escherichia coli using a non-induced expression system with different nutrient sources

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorVerdartis Desenvolvimento Biotecnol Ltda-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorFundacao Oswaldo Cruz-
Autor(es): dc.creatorMendonca, Elenira H. M.-
Autor(es): dc.creatorAvanci, Nilton Cesar-
Autor(es): dc.creatorRomano, Luis Henrique-
Autor(es): dc.creatorBranco, Daniel Lopes-
Autor(es): dc.creatorPadua, Alessandra Xavier de-
Autor(es): dc.creatorWard, Richard John-
Autor(es): dc.creatorBaptista Neto, Alvaro de [UNESP]-
Autor(es): dc.creatorLourenzoni, Marcos Roberto-
Data de aceite: dc.date.accessioned2022-02-22T00:05:22Z-
Data de disponibilização: dc.date.available2022-02-22T00:05:22Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s43153-019-00004-x-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195274-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195274-
Descrição: dc.descriptionThe application of enzymes for sustainable and low-environmental impact industrial processes requires high-level enzyme production at low-cost. A promising strategy is the use of a high efficiency heterologous protein expression system using E. coli and the pT7BsXA vector encoding the GH11 xylanase from Bacillus subtilis with promoter, replication origin and signal peptide sequences from B. subtilis (Ruller et al. 2006). This expression system produces high amounts of enzyme that are secreted to the culture broth. The present study aimed to maximize the xylanase production by this system through evaluation of culture medium composition. Different culture media previously described in the literature together with compositions derived from agro-industrial residues were evaluated. A culture medium derived from agro-industrial residues using sugarcane molasses as carbon source showed a 9-fold increase in enzyme production (195,000 U/L) in relation to LB medium and the lowest production cost, which was 8.5-fold lower than LB medium using sugarcane molasses as carbon source and brewer's yeast as vitamin source in shaker experiments. In a bioreactor experiment the best production medium promoted an 8.5-fold higher production at a 10.8-fold lower cost as compared to shaker LB cultivation.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionVerdartis Desenvolvimento Biotecnol Ltda, Av Dra Naldir Ribeirao Preto,1805,Jardim Dr Paulo, BR-14056680 Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3-900, BR-14040900 Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Bioprocessos & Biotecnol, Rodovia Araraquara Jau,Km 01,Campos Ville, BR-14800903 Araraquara, SP, Brazil-
Descrição: dc.descriptionFundacao Oswaldo Cruz, FIOCRUZ, Rua Sao Jose S-N, BR-61760000 Eusebio, CE, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Bioprocessos & Biotecnol, Rodovia Araraquara Jau,Km 01,Campos Ville, BR-14800903 Araraquara, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2008/53426-7-
Descrição: dc.descriptionFAPESP: 2010/50328-4-
Formato: dc.format29-39-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationBrazilian Journal Of Chemical Engineering-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectXylanase-
Palavras-chave: dc.subjectRecombinant enzymes-
Palavras-chave: dc.subjectBioprocess-
Palavras-chave: dc.subjectAgro-industrial residues-
Palavras-chave: dc.subjectE-
Palavras-chave: dc.subjectcoli-
Título: dc.titleRecombinant xylanase production by Escherichia coli using a non-induced expression system with different nutrient sources-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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