Strategies for aquaculture and conservation of Neotropical catfishes based on the production of triploid Pimelodus maculatus

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorChico Mendes Inst Biodivers Conservat-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorBertolini, Rafaela Manchin [UNESP]-
Autor(es): dc.creatorLopez, Lucia Suarez [UNESP]-
Autor(es): dc.creatorNascimento, Nivaldo Ferreira do-
Autor(es): dc.creatorArashiro, Dilberto Ribeiro [UNESP]-
Autor(es): dc.creatorSiqueira Silva, Diogenes Henrique de-
Autor(es): dc.creatorAlves dos Santos, Silvio Carlos-
Autor(es): dc.creatorSenhorini, Jose Augusto [UNESP]-
Autor(es): dc.creatorYasui, George Shigueki [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:05:13Z-
Data de disponibilização: dc.date.available2022-02-22T00:05:13Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10499-019-00449-4-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195221-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195221-
Descrição: dc.descriptionTriploidization is a technique to produce sterile fish on a large scale. In the spotted catfish Pimelodus maculatus, sterile fish serve as a host of germ cell transplantation for subsequent reconstitution of endangered catfish species. This study aimed to establish an efficient protocol for triploidization in P. maculatus using temperature shock. In this study, we tested the efficacy of heat shock treatments (37 degrees C, 38 degrees C, and 39 degrees C) applied at 2 min post-fertilization (mpf) for the duration of 2 min. Intact embryos served as diploid control. Ploidy status was confirmed by flow cytometry, characterization of erythrocytes nuclei, and karyotyping. Developmental stages were verified at cleavage, blastula, gastrula, somite, and hatching stages, as well as the percentages of normal and abnormal larvae. No significant difference was observed between the temperature treatments for percentage of unfertilized oocytes (P = 0.1834), 2-cell stage (P = 0.2348), blastula (P = 0.2972), hatching (P = 0.0668), and abnormal larvae (P = 0.6177). Heat shock decreased the survival at the blastula stage (P = 0.0178), somite (P = 0.0469), and the percentage of abnormal larvae (P = 0.0261). All treatments presented high percentages of triploid individuals, and the highest values were observed for heat shock at 38 degrees C (96.7 %). Based on the results described above, an efficient protocol for triploidization in P. maculatus was established using heat shock (38 degrees C) at 2 mpf for 2 min.-
Descrição: dc.descriptionAES Tiete/ANEEL-
Descrição: dc.descriptionChico Mendes Inst Biodivers Conservat, Natl Ctr Res & Conservat Continental Fish, Lab Fish Biotechnol, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, Rua Prof Doutor Antonio Celso Wagner Zanin S-No, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, Rua Prof Doutor Antonio Celso Wagner Zanin S-No, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionAES Tiete/ANEEL: 4690000174-
Formato: dc.format127-137-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationAquaculture International-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectSpotted catfish-
Palavras-chave: dc.subjectChimera-
Palavras-chave: dc.subjectChromosome manipulation-
Palavras-chave: dc.subjectPolyploidy-
Palavras-chave: dc.subjectTriploidization-
Título: dc.titleStrategies for aquaculture and conservation of Neotropical catfishes based on the production of triploid Pimelodus maculatus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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