miR156-targeted SPL10 controls Arabidopsis root meristem activity and root-derived de novo shoot regeneration via cytokinin responses

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Roma La Sapienza-
Autor(es): dc.contributorUniversidade Federal de Viçosa (UFV)-
Autor(es): dc.creatorBarrera-Rojas, Carlos Hernan [UNESP]-
Autor(es): dc.creatorBraga Rocha, Gabriel Henrique-
Autor(es): dc.creatorPolverari, Laura-
Autor(es): dc.creatorPinheiro Brito, Diego Armando-
Autor(es): dc.creatorBatista, Diego Silva-
Autor(es): dc.creatorNotini, Marcela M.-
Autor(es): dc.creatorFerreira da Cruz, Ana Claudia-
Autor(es): dc.creatorOrtiz Morea, Edna Gicela [UNESP]-
Autor(es): dc.creatorSabatini, Sabrina-
Autor(es): dc.creatorOtoni, Wagner Campos-
Autor(es): dc.creatorSilveira Nogueira, Fabio Tebaldi-
Data de aceite: dc.date.accessioned2022-02-22T00:05:12Z-
Data de disponibilização: dc.date.available2022-02-22T00:05:12Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-01-22-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/jxb/erz475-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195217-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195217-
Descrição: dc.descriptionRoot growth is modulated by different factors, including phytohormones, transcription factors, and microRNAs (miRNAs). MicroRNA156 and its targets, the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes, define an age-dependent pathway that controls several developmental processes, including lateral root emergence. However, it remains unclear whether miR156-regulated SPLs control root meristem activity and root-derived de novo shoot regeneration. Here, we show that MIR156 and SPL genes have opposing expression patterns during the progression of primary root (PR) growth in Arabidopsis, suggesting that age cues may modulate root development. Plants with high miR156 levels display reduced meristem size, resulting in shorter primary root (PRs). Conversely, plants with reduced miR156 levels show higher meristem activity. Importantly, loss of function of SPL10 decreases meristem activity, while SPL10 de-repression increases it. Meristem activity is regulated by SPL10 probably through the reduction of cytokinin responses, via the modulation of type-B ARABIDOPSIS RESPONSE REGULATOR1(ARR1) expression. We also show that SPL10 de-repression in the PRs abolishes de novo shoot regenerative capacity by attenuating cytokinin responses. Our results reveal a cooperative regulation of root meristem activity and root-derived de novo shoot regeneration by integrating age cues with cytokinin responses via miR156-targeted SPL10.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Sao Paulo, Dept Biol Sci, Lab Mol Genet Plant Dev, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP, Brazil-
Descrição: dc.descriptionState Univ Sao Paulo, Biosci Inst, BR-18618970 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Roma La Sapienza, Lab Funct Genom & Prote Model Syst, Dipartimento Biol & Biotecnol, I-00185 Rome, Italy-
Descrição: dc.descriptionUniv Fed Vicosa, Dept Plant Biol, Plant Tissue Culture Lab BIOAGRO, BR-36570900 Vicosa, MG, Brazil-
Descrição: dc.descriptionState Univ Sao Paulo, Biosci Inst, BR-18618970 Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 15/17892-7-
Descrição: dc.descriptionFAPESP: 18/17441-3-
Formato: dc.format934-950-
Idioma: dc.languageen-
Publicador: dc.publisherOxford Univ Press-
Relação: dc.relationJournal Of Experimental Botany-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectArabidopsis thaliana-
Palavras-chave: dc.subjectde novo shoot regeneration-
Palavras-chave: dc.subjectmeristem-
Palavras-chave: dc.subjectmicroRNA156-
Palavras-chave: dc.subjectroot-
Palavras-chave: dc.subjectSPLs-
Título: dc.titlemiR156-targeted SPL10 controls Arabidopsis root meristem activity and root-derived de novo shoot regeneration via cytokinin responses-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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