Assessment of biophysical properties of Haemonchus contortus from different life cycle stages with atomic force microscopy

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Fed Maranhao-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorFed Inst Bahia-
Autor(es): dc.creatorCosta-Junior, Livio M.-
Autor(es): dc.creatorSilva, Carolina R.-
Autor(es): dc.creatorSoares, Alexandra M. S.-
Autor(es): dc.creatorMenezes, Alan S.-
Autor(es): dc.creatorSilva, Maria R. L. [UNESP]-
Autor(es): dc.creatorAmarante, Alessandro F. T. [UNESP]-
Autor(es): dc.creatorCosta, Erivelton F.-
Autor(es): dc.creatorAlencar, Luciana M. R.-
Data de aceite: dc.date.accessioned2022-02-22T00:04:59Z-
Data de disponibilização: dc.date.available2022-02-22T00:04:59Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ultramic.2019.112862-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195172-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195172-
Descrição: dc.descriptionAtomic force microscopy (AFM) was used in this work to investigate the ultrastructural and mechanical characteristics of Haemonchus contortus, the major gastrointestinal nematode that infects small ruminants worldwide. The biophysical characterization of this species is extremely important in order to reveal mechanisms of action of drugs and to classify its ultrastructure and biomechanical properties. High-resolution topographic images by AFM as well as data on biomechanical properties of cuticles were obtained at different stages of H. contortus. The results reveal details of the mechanical and structural properties of this nematode never observed before for nematodes parasite with other microscope techniques. Qualitative and quantitative reductions in the elasticity of the larvae stage egg were compared with those of the morulae stage, and the increased adhesion of unsheathed L(3 )were compared with the same stage of sheathed larvae. The results presented here open possibilities for understanding the mechanisms of drug and biomolecular actions that can be used to control infections caused by H. contortus.-
Descrição: dc.descriptionIECT Biotechnology through FAPEMA (Maranhao State Research Foundation, Brazil)-
Descrição: dc.descriptionFINEP (Brazilian Innovation Agency)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniv Fed Maranhao, Ctr Biol & Hlth Sci, Dept Pathol, Lab Parasite Control, Sao Luis, Maranhao, Brazil-
Descrição: dc.descriptionUniv Fed Maranhao, Ctr Exact Sci & Technol, Chem Engn Course, Sao Luis, Maranhao, Brazil-
Descrição: dc.descriptionUniv Fed Maranhao, Dept Phys, Sao Luis, Maranhao, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Biosci Inst, Botucatu, SP, Brazil-
Descrição: dc.descriptionFed Inst Bahia, IFBA, Irece, BA, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Biosci Inst, Botucatu, SP, Brazil-
Formato: dc.format9-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationUltramicroscopy-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectHaemonchus contortus-
Palavras-chave: dc.subjectAFM ultramicrography-
Palavras-chave: dc.subjectBiomechanics-
Título: dc.titleAssessment of biophysical properties of Haemonchus contortus from different life cycle stages with atomic force microscopy-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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