Extracellular Tannase from Aspergillus ochraceus: Influence of the Culture Conditions on Biofilm Formation, Enzyme Production, and Application

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorAracri, Fernanda Mansano [UNESP]-
Autor(es): dc.creatorFarias Cavalcanti, Rayza Morganna [UNESP]-
Autor(es): dc.creatorSouza Guimaraes, Luis Henrique-
Data de aceite: dc.date.accessioned2022-02-22T00:04:31Z-
Data de disponibilização: dc.date.available2022-02-22T00:04:31Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.4014/jmb.1903.03060-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/195006-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/195006-
Descrição: dc.descriptionAspergillus ochraceus biofilm, developed on an inert support, can produce tannase in Khanna medium containing 1.5% (w/v) tannic acid as the carbon source, at an initial pH of 5.0, for 72 h at 28 degrees C. Addition of 0.1% (w/v) yeast extract increased enzyme production. The enzyme in the crude filtrate exhibited the highest activity at 30 degrees C and pH 6.0. At 50 degrees C, the half-life was 60 min and 260 min at pH 6.0. In general, addition of detergents and surfactants did not affect tannase activity significantly. Tannase has potential applications in various biotechnological processes such as the production of propyl gallate and in the treatment of tannin-rich effluents. The content of tannins and total phenolic compounds in effluents from leather treatment was reduced by 56-83% and 47-64%, respectively, after 2 h of enzyme treatment. The content of tannins and total phenolic compounds in the sorghum flour treated for 120 h with tannase were reduced by 61% and 17%, respectively. Interestingly, the same A. ochraceus biofilm was able to produce tannase for three sequential fermentative process. In conclusion, fungal biofilm is an interesting alternative to produce high levels of tannase with biotechnological potential to be applied in different industrial sectors.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUNESP, Inst Quim Araraquara, Ave Prof Mario Degni S-N, BR-14800900 Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ave Bandeirantes 3900, Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionUNESP, Inst Quim Araraquara, Ave Prof Mario Degni S-N, BR-14800900 Araraquara, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2016/11311-5-
Formato: dc.format1749-1759-
Idioma: dc.languageen-
Publicador: dc.publisherKorean Soc Microbiology & Biotechnology-
Relação: dc.relationJournal Of Microbiology And Biotechnology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectBiofilm fermentation-
Palavras-chave: dc.subjectfungal biofilm-
Palavras-chave: dc.subjectleather effluent-
Palavras-chave: dc.subjecttannase-
Palavras-chave: dc.subjecttannin acyl hydrolase-
Título: dc.titleExtracellular Tannase from Aspergillus ochraceus: Influence of the Culture Conditions on Biofilm Formation, Enzyme Production, and Application-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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