Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorSakamoto, Isabel Kimiko-
Autor(es): dc.creatorMaintiguer, Sandra Imaculada [UNESP]-
Autor(es): dc.creatorAmancio Varesche, Maria Bernadete-
Data de aceite: dc.date.accessioned2022-02-22T00:04:25Z-
Data de disponibilização: dc.date.available2022-02-22T00:04:25Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.4025/actascitechnol.v41i1.39128-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/194969-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/194969-
Descrição: dc.descriptionGranulated sludge from anaerobic reactors is constituted by the microbial consortia responsible for the degradation of different substrate present in wastewaters. This study characterized anaerobic microorganisms in a granular sludge from a Uasb reactor (Upflow Anaerobic Sludge Blanket) by Most Probable Number (MPN) technique and method of cloning and sequencing the 16S rDNA gene. The main objective of this study was to quantify and to identify the microorganisms in two different culture media containing sulfate (Posgate C [6.0 sodium lactate and 3.13 g L-1 sodium sulfate] and Zinder [2.24 sodium lactate and 0.96 g L-1 sodium sulfate]). Microorganisms quantified by Postgate C and Zinder media were: 9.30x10(10) and 7.50x10(11) MPN mL(-1) for general anaerobic bacteria; 4.30x10(8) and 2.10x10(8) MPN mL(-1) for sulfate reducing bacteria; and 1.20x10(8) and 1.5x10(8) MPN mL(-1) for methane producing archaea, respectively. Bacteria identified by 16S rDNA gene sequencing from the MPN in both culture media were related to the phyla Firmicutes and Proteobacteria. The conditions of Postgate C culture medium favored the sulfate-reducing bacteria and the Zinder culture medium favored the general anaerobic bacteria.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionBrazilian Innovation Agency (Finep)-
Descrição: dc.descriptionUniv Sao Paulo, Dept Hidraul & Saneamento, Escola Engn Sao Carlos, Ave Joao Dagnone 1100, BR-13563120 Sao Paulo, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Pesquisa Bioenergia, Rio Claro, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Inst Pesquisa Bioenergia, Rio Claro, SP, Brazil-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherUniv Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao-
Relação: dc.relationActa Scientiarum-technology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectsulfate reducing bacteria-
Palavras-chave: dc.subjectmethane producing archaea-
Palavras-chave: dc.subject16S rDNA gene-
Palavras-chave: dc.subjectcloning/sequencing-
Palavras-chave: dc.subjectupflow anaerobic sludge blanket reactor-
Título: dc.titlePhylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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