Use of the aqueous biphasic system as an alternative for concentration of Ascaris lumbricoides eggs, with non-toxic separation of faecal residues and fats

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorRosa, Stefany Laryssa-
Autor(es): dc.creatorSantos, Bianca Martins dos-
Autor(es): dc.creatorSoares, Felipe Augusto-
Autor(es): dc.creatorNery Loiola, Saulo Hudson-
Autor(es): dc.creatorInacio, Sandra Valeria [UNESP]-
Autor(es): dc.creatorNagase Suzuki, Celso Tetsuo-
Autor(es): dc.creatorSabadini, Edvaldo-
Autor(es): dc.creatorFalcao, Alexandre Xavier-
Autor(es): dc.creatorSaraiva Bresciani, Katia Denise [UNESP]-
Autor(es): dc.creatorGomes, Jancarlo Ferreira-
Data de aceite: dc.date.accessioned2022-02-22T00:04:23Z-
Data de disponibilização: dc.date.available2022-02-22T00:04:23Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/tmi.13308-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/194957-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/194957-
Descrição: dc.descriptionObjectives Human enteroparasites are considered a serious public health problem in underdeveloped countries located in world regions with tropical, subtropical and equatorial climates. These parasites are commonly diagnosed by the Parasitological Examination of Faeces (PEF), performed by conventional techniques and/or commercial kits that result in tests with low-to-moderate sensitivity, due to the use of destructive chemical solvents to parasite structures, and to present excess adipose substance and digestive residues in their microscopic slides. In order to improve the efficacy of these tests/examinations, this study aimed to investigate a new alternative for the PEF, with the use of Aqueous Biphasic System (ABS). Methods For this, four ABSs containing poly (ethylene glycol), PEG (PEG-4000 and PEG-6000), dipotassium phosphate and sodium citrate at different concentrations in the biphasic systems were evaluated with faecal samples containing eggs of Ascaris lumbricoides. Results The ABS consisting of PEG-4000 and dipotassium phosphate, at concentrations of 55% w/w and 20% w/w, respectively, achieved 100% satisfactory results compared to the conventional TF-Test technique in terms of preservation and concentration of A. lumbricoides eggs, with adequate separation of digestive residues, without using a centrifuge or chemical solvents that may cause harm to the parasites. Conclusions This study presents ABS as a new low-cost technical principle for the detection of parasite eggs in PEF. The new technique is simple, fast, non-toxic, not harmful to the parasite and does not require a centrifuge.-
Descrição: dc.descriptionUniv Estadual Campinas, Sch Med Sci, Rua Saturnino Brito,573,Room 364, BR-13083852 Campinas, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Vet Med, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, Inst Comp, Campinas, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, Inst Chem, Campinas, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Vet Med, Sao Paulo, Brazil-
Formato: dc.format1320-1329-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell-
Relação: dc.relationTropical Medicine & International Health-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectenteroparasites-
Palavras-chave: dc.subjectaqueous biphasic system-
Palavras-chave: dc.subjectdiagnosis-
Título: dc.titleUse of the aqueous biphasic system as an alternative for concentration of Ascaris lumbricoides eggs, with non-toxic separation of faecal residues and fats-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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