Immunohistochemical and Molecular Diagnosis of Mucocutaneous and Mucosal Leishmaniasis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniv Republica-
Autor(es): dc.contributorUniv Estadual Montes Claros-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Fed Juiz de Fora-
Autor(es): dc.creatorSanchez-Romero, Celeste-
Autor(es): dc.creatorMartelli Junior, Hercilio-
Autor(es): dc.creatorRibeiro da Matta, Vania Lucia [UNESP]-
Autor(es): dc.creatorFreitas, Larisse Miranda-
Autor(es): dc.creatorSoares, Carina de Mattos [UNESP]-
Autor(es): dc.creatorMariano, Fernanda Viviane [UNESP]-
Autor(es): dc.creatorAlmeida, Oslei Paes de-
Autor(es): dc.creatorAquino, Sibele Nascimento de-
Data de aceite: dc.date.accessioned2022-02-22T00:04:13Z-
Data de disponibilização: dc.date.available2022-02-22T00:04:13Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2019-09-29-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1177/1066896919876706-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/194899-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/194899-
Descrição: dc.descriptionLeishmaniasis is a parasitic infection transmitted by the bite of infected female sandflies. It principally affects the skin, and the frequency of mucosal involvement is about 5% to 20%. Considering the rarity of leishmaniasis affecting the upper aerodigestive tract mucosa, we evaluated the characteristics of mucocutaneous leishmaniasis and mucosal leishmaniasis and the diagnostic difficulty when the parasites are scarce in tissue samples. The clinical, histopathological, histochemical, immunohistochemical, and molecular features of 17 cases of mucocutaneous leishmaniasis and mucosal leishmaniasis were assessed. Mucosal disease was principally found in the soft palate, oropharynx, and nose, manifesting mainly as a solitary ulcer. In hematoxylin and eosin-stained sections, 10 cases revealed abundant amastigotes within the macrophages. Giemsa staining was not shown to be helpful to confirm the diagnosis in 6 cases with scarce or nondetectable amastigotes. Immunohistochemistry (IHC) showed high sensitivity by positive staining in 14 out of 17 cases (82.3%). Polymerase chain reaction was shown to be more sensitive than IHC with 13 out of 14 (92.8%) positive cases, including the 3 IHC negative cases; however, this technique is not available in many endemic regions. In summary, we suggest that the IHC is a simple technique with rapid results and relatively low cost, when compared with other laboratorial procedures; thus, IHC is a helpful tool that should be implemented in the routine diagnosis of leishmania.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionUniv Estadual Campinas, UNICAMP, Piracicaba, Brazil-
Descrição: dc.descriptionUniv Republica, Montevideo, Uruguay-
Descrição: dc.descriptionUniv Estadual Montes Claros, Montes Claros, MG, Brazil-
Descrição: dc.descriptionState Univ Sao Paulo USP, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Fed Juiz de Fora, Governador Valadares, MG, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, UNICAMP, Campinas, SP, Brazil-
Descrição: dc.descriptionState Univ Sao Paulo USP, Sao Paulo, Brazil-
Descrição: dc.descriptionFAPEMIG: APQ01122/14-
Formato: dc.format138-145-
Idioma: dc.languageen-
Publicador: dc.publisherSage Publications Inc-
Relação: dc.relationInternational Journal Of Surgical Pathology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectmucocutaneous leishmaniasis-
Palavras-chave: dc.subjectoral mucosa-
Palavras-chave: dc.subjectnasal mucosa-
Palavras-chave: dc.subjectimmunohistochemistry-
Palavras-chave: dc.subjectpolymerase chain reaction-
Título: dc.titleImmunohistochemical and Molecular Diagnosis of Mucocutaneous and Mucosal Leishmaniasis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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