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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual de Campinas (UNICAMP) | - |
Autor(es): dc.contributor | Univ Vale Paraiba | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Monica, Fabiola Z. | - |
Autor(es): dc.creator | Rojas-Moscoso, Julio | - |
Autor(es): dc.creator | Porto, Marcovan | - |
Autor(es): dc.creator | Schenka, Andre A. | - |
Autor(es): dc.creator | Antunes, Edson | - |
Autor(es): dc.creator | Cogo, Jose Carlos | - |
Autor(es): dc.creator | De Nucci, Gilberto [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:03:43Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:03:43Z | - |
Data de envio: dc.date.issued | 2020-12-09 | - |
Data de envio: dc.date.issued | 2020-12-09 | - |
Data de envio: dc.date.issued | 2012-04-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.cbpc.2011.11.003 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/194734 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/194734 | - |
Descrição: dc.description | We characterized the nitric oxide (NO)-cyclic GMP-phosphodiesterase-5 (PDE5) pathway in Crotalus durissus terrificus aorta. Concentration responses curves to acetylcholine (ACh), sodium nitroprusside (SNP), BAY41-2272 (soluble guanylyl cyclase [sGC] stimulator), BAY60-2770 (sGC activator) and tadalafil (PDE5 inhibitor) were constructed in phenylephrine (10 mu M)-precontracted tissues with intact (E+) or denuded (E-) endothelium. ACh (0.0001-10 mu M) and SNP (0.0001-10 mu M) relaxed aorta, which were reduced by the NO synthase (L-NAME,100 mu M) or the sGC inhibitors (ODQ 10 mu M). Tadalafil (0.0001-10 mu M) relaxed E+ rings with potency (pEC(50)) and maximal response (E-max) values of 7.34 +/- 0.02 and 105 +/- 8%, respectively. E- or ODQ treatment significantly (P < 0.05) reduced tadalafil relaxations (66 +/- 18% and 71 +/- 7%, respectively). BAY41-2272 (0.0001-300 nM) produced concentration-dependent relaxations in E+ rings, which were reduced by addition of either ODQ or L-NAME (16.0- and 5.2-fold rightward shifts, respectively). The relaxation of BAY60-2770 was markedly potentiated by ODQ and L-NAME (41.0- and 9.7-fold leftward shifts, respectively), whereas in E- the pEC(50) values were shifted by 7-fold to the right. Immunohistochemistry, followed validation by transcriptomic analysis, revealed the presence of eNOS in endothelium, whereas nNOS was observed only in perivascular nerves. sGC and PDE5 were expressed in smooth muscle. Thus, NO-sGC-PDE5 pathway is evolutionarily present in Crotalus sp. vessels, and has a remarkable degree of functional similarity to mammalian vessels. (C) 2011 Elsevier Inc. All rights reserved. | - |
Descrição: dc.description | State Univ Campinas UNICAMP, Dept Pharmacol, Fac Med Sci, Campinas, SP, Brazil | - |
Descrição: dc.description | Univ Vale Paraiba, Inst Res & Dev, Lab Inflammat, Sao Jose Dos Campos, SP, Brazil | - |
Descrição: dc.description | State Univ Sao Paulo USP, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP, Brazil | - |
Descrição: dc.description | State Univ Sao Paulo USP, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP, Brazil | - |
Formato: dc.format | 433-439 | - |
Idioma: dc.language | en | - |
Publicador: dc.publisher | Elsevier B.V. | - |
Relação: dc.relation | Comparative Biochemistry And Physiology C-toxicology & Pharmacology | - |
???dc.source???: dc.source | Web of Science | - |
Palavras-chave: dc.subject | Aorta | - |
Palavras-chave: dc.subject | Crotalus durissus | - |
Palavras-chave: dc.subject | Nitric oxide synthase | - |
Palavras-chave: dc.subject | Phosphodiesterase type 5 | - |
Palavras-chave: dc.subject | Soluble guanylyl cyclase | - |
Título: dc.title | Immunohistochemical and functional characterization of nitric oxide signaling pathway in isolated aorta from Crotalus durissus terrificus | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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