Enzymatic and oxidative metabolites of lycopene (vol 14, pg 531, 2003)

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Autor(es): dc.contributorTufts Univ-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFerreira, ALD-
Autor(es): dc.creatorYeum, K. J.-
Autor(es): dc.creatorRussell, R. M.-
Autor(es): dc.creatorKrinsky, N. I.-
Autor(es): dc.creatorTang, G. W.-
Data de aceite: dc.date.accessioned2022-02-22T00:03:27Z-
Data de disponibilização: dc.date.available2022-02-22T00:03:27Z-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2020-12-09-
Data de envio: dc.date.issued2004-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/S0955-2863(03)001074-4-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/194630-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/194630-
Descrição: dc.descriptionUsing the post-mitochondrial fraction of rat intestinal mucosa, we have investigated lycopene metabolism. The incubation media was composed of NAD(+), KCl, and DTT with or without added lipoxygenase. The addition of lipoxygenase into the incubation significantly increased the production of lycopene metabolites. The enzymatic incubation products of H-2(10) lycopene were separated using high-performance liquid chromatography and analyzed by UV/Vis spectrophotometer and atmospheric pressure chemical ionization-mass spectroscopy. We have identified two types of products: cleavage products and oxidation products. The cleavage products are likely: (1) 3-keto-apo-13-lycopenone (C18H24O2 or 6,10,14-trimethyl-12-one-3,5,7,9,13-pentadecapentaen-2-one) with lambdamax = 365 nm and m/z = 272 and (2) 3,4-dehydro-5,6-dihydro-15-apo-lycopenal (C20H28O or 3,7,11,15-tetramethyl-2,4,6,8,12,14-hexadecahexaen-1-al) with lambdamax = 380 nm and m/z = 284. The oxidative metabolites are likely: (3) 2-ene-5,8-lycopenal-furanoxide (C37H50O) with lambdamax = 415 nm, 435 nm, and 470 nm, and m/z = 510; (4) lycopene-5, 6, 5', 6'-diepoxide (C40H56O2) with lambdamax = 415 nm, 440 nm, and 470 nm, and m/z = 568; (5) lycopene-5,8-furanoxide isomer (1) (C40H56O) with lambdamax = 410 nm, 440 nm, and 470 nm, and m/z = 552; (6) lycopene-5,8-epoxide isomer (II) (C40H56O) with lambdamax = 410, 440, 470 nm, and m/z = 552; and (7) 3-keto-lycopene-5',8'-furanoxide (C40H54O2) with lambdamax = 400 nm, 420 nm, and 450 nm, and m/z = 566. These results demonstrate that both central and excentric cleavage of lycopene occurs in the rat intestinal mucosa in the presence of soy lipoxygenase. (C) 2003 Elsevier Inc. All rights reserved.-
Descrição: dc.descriptionTufts Univ, Human Nutr Res Ctr Aging, USDA, Boston, MA 02111 USA-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Med, BR-18618970 Botucatu, SP, Brazil-
Descrição: dc.descriptionTufts Univ, Sch Med, Dept Biochem, Boston, MA 02111 USA-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Med, BR-18618970 Botucatu, SP, Brazil-
Formato: dc.format493-502-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationJournal Of Nutritional Biochemistry-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectlycopene-
Palavras-chave: dc.subjectrat-
Palavras-chave: dc.subjectmetabolites-
Palavras-chave: dc.subjectoxidation-
Palavras-chave: dc.subjectcleavage-
Palavras-chave: dc.subjectenzyme-
Título: dc.titleEnzymatic and oxidative metabolites of lycopene (vol 14, pg 531, 2003)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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