Diversity of Anaplasma species in cattle in Mozambique

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFernandes, Simone de Jesus-
Autor(es): dc.creatorMatos, Carlos António-
Autor(es): dc.creatorFreschi, Carla Roberta-
Autor(es): dc.creatorde Souza Ramos, Inalda Angélica-
Autor(es): dc.creatorMachado, Rosangela Zacarias-
Autor(es): dc.creatorAndré, Marcos Rogério-
Data de aceite: dc.date.accessioned2021-03-11T01:40:56Z-
Data de disponibilização: dc.date.available2021-03-11T01:40:56Z-
Data de envio: dc.date.issued2019-10-06-
Data de envio: dc.date.issued2019-10-06-
Data de envio: dc.date.issued2019-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2019.02.012-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/190148-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/190148-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Goiás-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Goiás: 2015/14896-1-
Descrição: dc.descriptionAlthough species of Anaplasma are highly prevalent Rickettsiales agents in domestic and wild ruminants with a wide distribution worldwide, few studies have been conducted so far to detect and/or investigate the diversity of these agentsin cattle in Mozambique. In the present study, serological and molecular assays were used to investigate the occurrence of Anaplasma spp. in 219 bovines sampled in the districts of Boane, Magude, Matutuíne, Moamba and Namaacha in Maputo, Mozambique. In the iELISA test for detection ofIgG antibodies to A. marginale, 86.3% (189/219) of the samples were positive. In qPCR assays for the gene msp1β for A. marginale and msp2 for A. phagocytophilum, 97.3% (213/219) and 2.7% (6/219) of the animals were positive, respectively. Two different cPCR protocols based on the 16S rRNA gene showed that 100% of the samples were positive for Anaplasma spp. The DNA sequences obtained were phylogenetically related to A. platys, A. phagocytophilum, Candidatus Anaplasma boleense, A. centrale, A. marginale and A. ovis. Phylogenetic inference based on the msp4 and msp5 genes positioned the obtained sequences in the clade of A. marginale, with evidence of occurrence of 8 and 5 different haplotypes for each gene, respectively. Anaplasma sp. phylogenetically associated with A. platys was evidenced in phylogenetic analyzes based on 16S rRNA and groEL genes. It is concluded that a high diversity of species of Anaplasma spp. occurs in cattle in Mozambique.-
Formato: dc.format651-664-
Idioma: dc.languageen-
Relação: dc.relationTicks and Tick-borne Diseases-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectAnaplasma centrale-
Palavras-chave: dc.subjectAnaplasma marginale-
Palavras-chave: dc.subjectAnaplasma ovis-
Palavras-chave: dc.subjectAnaplasma phagocytophilum-
Palavras-chave: dc.subjectAnaplasma platys-
Palavras-chave: dc.subject‘Candidatus Anaplasma boleense’-
Título: dc.titleDiversity of Anaplasma species in cattle in Mozambique-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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