Preliminary study on testicular germ cell isolation and transplantation in an endangered endemic species Brycon orbignyanus (Characiformes: Characidae)

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Siqueira-Silva, Diógenes Henrique-
Autor(es): dc.creatordos Santos Silva, Amanda Pereira-
Autor(es): dc.creatorda Silva Costa, Raphael-
Autor(es): dc.creatorSenhorini, José Augusto-
Autor(es): dc.creatorNinhaus-Silveira, Alexandre-
Autor(es): dc.creatorVeríssimo-Silveira, Rosicleire-
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Descrição: dc.descriptionWe aimed to develop a simplified protocol for transplantation of Brycon orbignyanus spermatogonial stem cells (SSCs) into Astyanax altiparanae testes. Brycon orbignyanus testes were enzymatically digested and SSC purified by a discontinuous density gradient. Endogenous spermatogenesis was suppressed in A. altiparanae using busulfan or by incubation at 35 °C water, and SSCs from B. orbignyanus labeled with PKH26 were injected into their testes via the urogenital papilla. Twenty-two hours post-transplantation, labeled spermatogonia were observed in A. altiparanae tubular lumen. After 7 days, spermatogonia proliferated in the epithelium, and 21 days post-transplantation, sperm was observed in the lumen. Of surviving host fish, nearly 67% of those treated with busulfan and 85% of those held in warm water showed labeled cells in host germinal epithelium. The present study standardized, by a simple and accessible method, germ cell transplantation between sexually mature Characiformes fish species. This is the first report of xenogenic SSC transplantation in this fish order.-
Idioma: dc.languageen-
Relação: dc.relationFish Physiology and Biochemistry-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectFish chimera-
Palavras-chave: dc.subjectGerm cell technology-
Palavras-chave: dc.subjectSpermatogonial proliferation-
Palavras-chave: dc.subjectSurrogate production-
Título: dc.titlePreliminary study on testicular germ cell isolation and transplantation in an endangered endemic species Brycon orbignyanus (Characiformes: Characidae)-
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Aparece nas coleções:Repositório Institucional - Unesp

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