Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae)

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMachado, Tatiana C.-
Autor(es): dc.creatorPansonato-Alves, Jose C.-
Autor(es): dc.creatorPucci, Marcela B.-
Autor(es): dc.creatorNogaroto, Viviane-
Autor(es): dc.creatorAlmeida, Mara C.-
Autor(es): dc.creatorOliveira, Claudio-
Autor(es): dc.creatorForesti, Fausto-
Autor(es): dc.creatorBertollo, Luiz A. C.-
Autor(es): dc.creatorMoreira-Filho, Orlando-
Autor(es): dc.creatorArtoni, Roberto F.-
Autor(es): dc.creatorVicari, Marcelo R.-
Data de aceite: dc.date.accessioned2021-03-10T17:12:27Z-
Data de disponibilização: dc.date.available2021-03-10T17:12:27Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2011-07-25-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/1471-2156-12-65-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/18734-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/18734-
Descrição: dc.descriptionFundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionBackground: The Characidium (a Neotropical fish group) have a conserved diploid number (2n = 50), but show remarkable differences among species and populations in relation to sex chromosome systems and location of nucleolus organizer regions (NOR). In this study, we isolated a W-specific probe for the Characidium and characterized six Characidium species/populations using cytogenetic procedures. We analyzed the origin and differentiation of sex and NOR-bearing chromosomes by chromosome painting in populations of Characidium to reveal their evolution, phylogeny, and biogeography.Results: A W-specific probe for efficient chromosome painting was isolated by microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) amplification of W chromosomes from C. gomesi. The W probe generated weak signals dispersed on the proto sex chromosomes in C. zebra, dispersed signals in both W and Z chromosomes in C. lauroi and, in C. gomesi populations revealed a proximal site on the long arms of the Z chromosome and the entire W chromosome. All populations showed small terminal W probe sites in some autosomes. The 18S rDNA revealed distinctive patterns for each analyzed species/population with regard to proto sex chromosome, sex chromosome pair, and autosome location.Conclusions: The results from dual-color fluorescence in situ hybridization (dual-color FISH) using W and 18S rDNA probes allowed us to infer the putative evolutionary pathways for the differentiation of sex chromosomes and NORs, from structural rearrangements in a sex proto-chromosome, followed by gene erosion and heterochromatin amplification, morphological differentiation of the sex chromosomal pair, and NOR transposition, giving rise to the distinctive patterns observed among species/populations of Characidium. Biogeographic isolation and differentiation of sex chromosomes seem to have played a major role in the speciation process in this group of fish.-
Formato: dc.format8-
Idioma: dc.languageen-
Publicador: dc.publisherBiomed Central Ltd.-
Relação: dc.relationBMC Genetics-
Relação: dc.relation2.469-
Relação: dc.relation1,160-
Direitos: dc.rightsopenAccess-
Título: dc.titleChromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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