Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBrisola, Maiara Cristiane-
Autor(es): dc.creatorCrecencio, Regiane Boaretto-
Autor(es): dc.creatorBitner, Dinael Simão-
Autor(es): dc.creatorFrigo, Angélica-
Autor(es): dc.creatorRampazzo, Luana-
Autor(es): dc.creatorStefani, Lenita Moura-
Autor(es): dc.creatorFaria, Gláucia Amorim-
Data de aceite: dc.date.accessioned2021-03-11T01:30:48Z-
Data de disponibilização: dc.date.available2021-03-11T01:30:48Z-
Data de envio: dc.date.issued2019-10-06-
Data de envio: dc.date.issued2019-10-06-
Data de envio: dc.date.issued2019-01-10-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.scitotenv.2018.07.438-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/186875-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/186875-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionThe objective of this study was to verify the presence of antimicrobial resistant strains of Escherichia coli in pig farms and to use it as a biomarker to evaluate phenotypic and genotypic profiles of antimicrobial susceptibility, as well as the presence of Extended Spectrum Beta-lactamases (ESBLs) and fluoroquinolone resistance genes. Several samples (n = 306) collected from swine farms (n = 100) of Southern Brazil were used for E. coli isolation: 103 of swine feces, 105 of water, and 98 of soil. E. coli isolates were submitted to the disk-diffusion test to verify their antimicrobial susceptibility, to disk-approximation test to detect ESBL-producers, and to PCR analysis to search for ESBLs genes (blaCTY-M2, blaSHV-1, blaTEM-1, blaCTX-M2, blaOXA-1, blaPSE-1) and quinolone resistance genes (qnrA, qnrB and qnrS). The percentage of E. coli isolates found in feces, water and soil samples was 66.02%, 30.48% and 35.71%, respectively. The highest percentages of resistance were obtained for sulfamethoxazole associated with trimethoprim (63.70%), colistin (45.19%) and enrofloxacin (39.26%). Regarding the levels of multidrug resistance, 37.04% of the isolates were resistant to three or more classes of antimicrobials. The most common profile (16%) of multirresistance was GEM-SUT-ENO-COL. The index of multiple resistance to antimicrobials (IRMA) was above 0.2 in 78% of the multiresistant isolates. Out of 135 E. coli isolates, 7.41% was ESBL-producers, of which 50% showed the blaCMY-M2 gene, 40% the blaTEM-1 and 70% the qnrS gene. Of non-ESBL-producing strains resistant to enrofloxacin, 13.04% were positives for qnrS gene. These results demonstrated the presence of fecal contamination in the environment, in addition to high resistance indexes for several antimicrobials, including beta-lactams and fluoroquinolones, which was confirmed by the genetic detection of ESBLs and qnr genes.-
Formato: dc.format362-368-
Idioma: dc.languageen-
Relação: dc.relationScience of the Total Environment-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectESBLs-
Palavras-chave: dc.subjectEscherichia coli-
Palavras-chave: dc.subjectFecal contamination-
Palavras-chave: dc.subjectFluoroquinolones-
Palavras-chave: dc.subjectResistance-
Título: dc.titleEscherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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