Myostatin and follistatin expression in skeletal muscles of rats with chronic heart failure

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorRuiz Lima, Aline Regina-
Autor(es): dc.creatorMartinez, Paula Felippe-
Autor(es): dc.creatorOkoshi, Katashi-
Autor(es): dc.creatorGuizoni, Daniele Mendes-
Autor(es): dc.creatorZornoff, Leonardo Antonio Mamede-
Autor(es): dc.creatorSalome Campos, Dijon Henrique-
Autor(es): dc.creatorOliveira, Silvio Assis-
Autor(es): dc.creatorBonomo, Camila-
Autor(es): dc.creatorDal Pai-Silva, Maeli-
Autor(es): dc.creatorOkoshi, Marina Politi-
Data de aceite: dc.date.accessioned2021-03-10T17:12:10Z-
Data de disponibilização: dc.date.available2021-03-10T17:12:10Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2010-02-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/j.1365-2613.2009.00683.x-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/18612-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/18612-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação para o Desenvolvimento da UNESP (FUNDUNESP)-
Descrição: dc.descriptionProcesso FAPESP: 06/61835-9-
Descrição: dc.descriptionProcesso FAPESP: 05/60767-7-
Descrição: dc.descriptionCNPq: 310547/2006-7-
Descrição: dc.descriptionCNPq: 309494/2006-0-
Descrição: dc.descriptionFUNDUNESP: 00108/06-DFp-
Descrição: dc.descriptionP>Skeletal muscle abnormalities can contribute to decreased exercise capacity in heart failure. Although muscle atrophy is a common alteration in heart failure, the mechanisms responsible for muscle mass reduction are not clear. Myostatin, a member of TGF-beta family (transforming growth factor), regulates muscle growth and mass. Several studies have shown a negative correlation between myostatin expression and muscle mass. The aim of this study was to evaluate myostatin expression in skeletal muscles of rats with heart failure. As myostatin gene expression can be modulated by follistatin, we also evaluated its expression. Heart failure was induced by myocardial infarction (MI, n = 10); results were compared to Sham-operated group (n = 10). Ventricular function was assessed by echocardiogram. Gene expression was analyzed by real-time PCR and protein levels by Western blotting in the soleus and gastrocnemius muscles; fibre trophism was evaluated by morphometric analysis. MI group presented heart failure evidence such as pleural effusion and right ventricular hypertrophy. Left ventricular dilation and dysfunction were observed in MI group. In the soleus muscle, cross-sectional area (P = 0.006) and follistatin protein levels (Sham 1.00 +/- 0.36; MI 0.18 +/- 0.06 arbitrary units; P = 0.03) were lower in MI and there was a trend for follistatin gene expression to be lower in MI group (P = 0.085). There was no change in myostatin expression between groups. In gastrocnemius, all MI group parameters were statistically similar to the Sham. In conclusion, our data show that during chronic heart failure, decreased skeletal muscle trophism is combined with unchanged myostatin and reduced follistatin expression.-
Formato: dc.format54-62-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell Publishing, Inc-
Relação: dc.relationInternational Journal of Experimental Pathology-
Relação: dc.relation1.938-
Relação: dc.relation0,712-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectcardiac failure-
Palavras-chave: dc.subjectfollistatin-
Palavras-chave: dc.subjectmyocardial infarction-
Palavras-chave: dc.subjectmyostatin-
Palavras-chave: dc.subjectPCR-
Palavras-chave: dc.subjectskeletal muscle-
Título: dc.titleMyostatin and follistatin expression in skeletal muscles of rats with chronic heart failure-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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