Genetic identification of the sharks Rhizoprionodon porosus and R. lalandii by PCR-RFLP and nucleotide sequence analyses of 5S rDNA

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPinhal, Danillo-
Autor(es): dc.creatorGadig, Otto Bismarck Fazzano-
Autor(es): dc.creatorMartins, Cesar-
Data de aceite: dc.date.accessioned2021-03-10T17:12:05Z-
Data de disponibilização: dc.date.available2021-03-10T17:12:05Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2009-12-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s12686-009-9008-9-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/18570-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/18570-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionA molecular approach based on nuclear 5S rDNA sequence variability was applied successfully to correctly identify samples from the two Rhizoprionodon species collected in the wild or sold in markets. The sequence of the non-transcribed spacer (NTS) of the 5S rDNA showed high interspecific variability and no intra-specific polymorphism, making it a useful marker for sharpnose shark identification. Polymorphisms in the NTS sequences of Rhizoprionodon sharks also created unique restriction patterns for each species after PCR-RFLP analysis. The 5S rDNA polymorphism represents a fast and non expensive tool to access species identification when rapid and unequivocal identification of shark products is needed, particularly for future management and other investigations.-
Formato: dc.format35-38-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationConservation Genetics Resources-
Relação: dc.relation0.742-
Relação: dc.relation0,430-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectGenetic markers-
Palavras-chave: dc.subjectNon-transcribed spacer-
Palavras-chave: dc.subjectTrade monitoring-
Palavras-chave: dc.subjectSharks-
Palavras-chave: dc.subjectSpecies identification-
Título: dc.titleGenetic identification of the sharks Rhizoprionodon porosus and R. lalandii by PCR-RFLP and nucleotide sequence analyses of 5S rDNA-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.